Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

The complexity of nanoscale interactions between biomaterials and cells has limited the
realization of the ultimate vision of nanotechnology in diagnostics and therapeutics. As such …

We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in
the fourth major release of the RELION software. In particular, we introduce VDAM, a …

Interpreting electron cryo-microscopy (cryo-EM) maps with atomic models requires high
levels of expertise and labour-intensive manual intervention in three-dimensional computer …

Here, we describe the third major release of RELION. CPU-based vector acceleration has
been added in addition to GPU support, which provides flexibility in use of resources and …

Single-particle cryo-electron tomography is an emerging technique capable of determining
the structure of proteins imaged within the native context of cells at molecular resolution …

The acquisition of cryo-electron microscopy (cryo-EM) data from biological specimens must
be tightly coupled to data preprocessing to ensure the best data quality and microscope …

Cryo-electron microscopy is a popular method for the determination of protein structures;
however, identifying a sufficient number of particles for analysis can take months of manual …

Parkinson's disease is a progressive neuropathological disorder that belongs to the class of
synucleinopathies, in which the protein alpha-synuclein is found at abnormally high …

Background Since the introduction of what became today's standard for cryo-embedding of
biological macromolecules at native conditions more than 30 years ago, techniques and …