[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
Epigenetic regulation of T cell exhaustion
Chronic antigen stimulation during viral infections and cancer can lead to T cell exhaustion,
which is characterized by reduced effector function and proliferation, and the expression of …
which is characterized by reduced effector function and proliferation, and the expression of …
The primordial differentiation of tumor-specific memory CD8+ T cells as bona fide responders to PD-1/PD-L1 blockade in draining lymph nodes
Q Huang, X Wu, Z Wang, X Chen, L Wang, Y Lu… - Cell, 2022 - cell.com
Summary Blocking PD-1/PD-L1 signaling transforms cancer therapy and is assumed to
unleash exhausted tumor-reactive CD8+ T cells in the tumor microenvironment (TME) …
unleash exhausted tumor-reactive CD8+ T cells in the tumor microenvironment (TME) …
Shared and distinct biological circuits in effector, memory and exhausted CD8+ T cells revealed by temporal single-cell transcriptomics and epigenetics
Naïve CD8+ T cells can differentiate into effector (Teff), memory (Tmem) or exhausted (Tex)
T cells. These developmental pathways are associated with distinct transcriptional and …
T cells. These developmental pathways are associated with distinct transcriptional and …
Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
T cell exhaustion limits antitumor immunity, but the molecular determinants of this process
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
A genome-scale screen for synthetic drivers of T cell proliferation
The engineering of autologous patient T cells for adoptive cell therapies has revolutionized
the treatment of several types of cancer. However, further improvements are needed to …
the treatment of several types of cancer. However, further improvements are needed to …
RASA2 ablation in T cells boosts antigen sensitivity and long-term function
The efficacy of adoptive T cell therapies for cancer treatment can be limited by suppressive
signals from both extrinsic factors and intrinsic inhibitory checkpoints,. Targeted gene editing …
signals from both extrinsic factors and intrinsic inhibitory checkpoints,. Targeted gene editing …
[HTML][HTML] The mitochondrial pyruvate carrier regulates memory T cell differentiation and antitumor function
M Wenes, A Jaccard, T Wyss, N Maldonado-Pérez… - Cell Metabolism, 2022 - cell.com
Glycolysis, including both lactate fermentation and pyruvate oxidation, orchestrates CD8+ T
cell differentiation. However, how mitochondrial pyruvate metabolism and uptake controlled …
cell differentiation. However, how mitochondrial pyruvate metabolism and uptake controlled …
Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
Clinical response to adoptive T cell therapies is associated with the transcriptional and
epigenetic state of the cell product. Thus, discovery of regulators of T cell gene networks and …
epigenetic state of the cell product. Thus, discovery of regulators of T cell gene networks and …
Stem-like exhausted and memory CD8+ T cells in cancer
T Gebhardt, SL Park, IA Parish - Nature Reviews Cancer, 2023 - nature.com
T cells can acquire a broad spectrum of differentiation states following activation. At the
extreme ends of this continuum are short-lived cells equipped with effector machinery and …
extreme ends of this continuum are short-lived cells equipped with effector machinery and …