Mammalian cells selected to resist N-(phosphonacetyl)-L-aspartate (PALA) contain
amplified copies of the CAD gene. While a single 7.9-kb mRNA species is detected in PALA …

Publisher Summary This chapter presents a detailed experimental procedure for a modified
RNase protection assay in which the polymerase chain reaction (PCR) technique is adapted …

This thesis reports the construction of genomic DNA libraries, using chromosomal DNA from
rodent derived cells which contain chromosomally integrated hepatitis B virus DNA …

ПРИМЕНЕНИЕ МЕТОДА ЗАЩИТЫ ОТ НУКЛЕАЗ ДЛЯ ИДЕНТИФИКАЦИИ ОТДЕЛЬНЫХ
МОЛЕКУЛ РНК Page 46 46 УДК 577.214 ЕЮ Шувалова студент 4-го курса Институт …

Metastatic melanoma is a devastating and poorly understood disease that is extremely
resistant to current approved therapeutic regimens. The earliest and most common observed …

In addition to the conserved AAUAAA hexanucleotide, GU and U-rich sequences in the
3$\sp\prime $ flanking region are thought to be critical for the efficient polyadenylation of …

In cells infected with avian sarcoma virus, 15 percent of the proviral mRNAs result from
readthrough of the viral poly (A) site. To examine the elements necessary for wild-type levels …

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Transient transfections of COS cells allowed analysis of the processing and accumulation of
transcripts from pSV2neo/mouse $\beta $-globin chimera. Apparent dominance of splicing …

An in vitro mRNA 3'processing system was established. Nuclear extracts capable of
supporting 3'processing in this system were prepared from both HeLa cells and K562 cells; …