Comparison of electrochemical immunosensors based on gold nano materials and immunoblot techniques for detection of histidine-tagged proteins in culture medium
M Wąsowicz, S Viswanathan, A Dvornyk… - Biosensors and …, 2008 - Elsevier
In this work, the direct electrochemical determination of poly-histidine tagged proteins using
immunosensor based on anti-His (C-term) antibody immobilized on gold electrodes …
immunosensor based on anti-His (C-term) antibody immobilized on gold electrodes …
Catalytic Cleavage of Amide C− N Bond: Scandium, Manganese, and Zinc Catalysts for Esterification of Amides
Amide C− N bonds are thermodynamically stable and their fission, such as by hydrolysis
and alcoholysis, is considered a long‐challenging organic reaction. In general …
and alcoholysis, is considered a long‐challenging organic reaction. In general …
Application of Ni (II)-assisted peptide bond hydrolysis to non-enzymatic affinity tag removal
E Kopera, A Belczyk-Ciesielska, W Bal - PLoS One, 2012 - journals.plos.org
In this study, we demonstrate a non-enzymatic method for hydrolytic peptide bond cleavage,
applied to the removal of an affinity tag from a recombinant fusion protein, SPI2-SRHWAP …
applied to the removal of an affinity tag from a recombinant fusion protein, SPI2-SRHWAP …
Influence of the amino acid side chain on peptide bond hydrolysis catalyzed by a dimeric Zr (IV)-substituted Keggin type polyoxometalate
HGT Ly, G Absillis, TN Parac-Vogt - New Journal of Chemistry, 2016 - pubs.rsc.org
Peptide bond hydrolysis of 18 different dipeptides, divided into four groups depending on
the nature of the amino acid side chain, by the dimeric Zr (IV)-substituted Keggin type …
the nature of the amino acid side chain, by the dimeric Zr (IV)-substituted Keggin type …
Selective peptide bond hydrolysis of cysteine peptides in the presence of Ni (II) ions
Recently, we described a sequence-specific R1-(Ser/Thr) peptide bond hydrolysis reaction
in peptides of a general sequence R1–(Ser/Thr)–Xaa–His–Zaa–R, which occurs in the …
in peptides of a general sequence R1–(Ser/Thr)–Xaa–His–Zaa–R, which occurs in the …
The C2H2 zinc finger transcription factors are likely targets for Ni(ii) toxicity
E Kurowska, J Sasin-Kurowska, A Bonna… - Metallomics, 2011 - academic.oup.com
Ni (ii) ions are able to hydrolyze Naa-(Ser/Thr) peptide bonds in Naa-(Ser/Thr)-Xaa-His-Zaa
sequences. We found that various human transcription factors contain such nickel hydrolytic …
sequences. We found that various human transcription factors contain such nickel hydrolytic …
Human annexins A1, A2, and A8 as potential molecular targets for Ni (II) ions
NE Wezynfeld, K Bossak, W Goch… - Chemical Research …, 2014 - ACS Publications
Nickel is harmful for humans, but molecular mechanisms of its toxicity are far from being fully
elucidated. One of such mechanisms may be associated with the Ni (II)-dependent peptide …
elucidated. One of such mechanisms may be associated with the Ni (II)-dependent peptide …
Sequence-specific Cu (II)-dependent peptide bond hydrolysis: similarities and differences with the Ni (II)-dependent reaction
A Belczyk-Ciesielska, IA Zawisza, M Mital… - Inorganic …, 2014 - ACS Publications
Potentiometry and UV–vis and circular dichroism spectroscopies were applied to
characterize Cu (II) coordination to the Ac-GASRHWKFL-NH2 peptide. Using HPLC and ESI …
characterize Cu (II) coordination to the Ac-GASRHWKFL-NH2 peptide. Using HPLC and ESI …
Sequence-specific Ni (II)-dependent peptide bond hydrolysis for protein engineering: Active sequence optimization
AM Protas, HHN Ariani, A Bonna… - Journal of inorganic …, 2013 - Elsevier
In previous studies we showed that Ni (II) ions can hydrolytically cleave a peptide bond
preceding Ser/Thr in peptides of a general sequence RN–(Ser/Thr)–Xaa–His–Zaa–RC …
preceding Ser/Thr in peptides of a general sequence RN–(Ser/Thr)–Xaa–His–Zaa–RC …
Metal complexes as artificial proteases in proteomics: A palladium (II) complex cleaves various proteins in solutions containing detergents
F Miskevich, A Davis, P Leeprapaiwong… - Journal of Inorganic …, 2011 - Elsevier
Most popular agents for site-specific protein cleavage are proteolytic enzymes. Because
they become denatured and inactivated by detergents, enzymes are inconvenient for …
they become denatured and inactivated by detergents, enzymes are inconvenient for …