Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and
cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and …

Trans-differentiation of human induced pluripotent stem cells into neurons (hiPSC-N) via
Ngn2-induction has become an efficient system to quickly generate neurons for disease …

ELAV/Hu factors are conserved RNA binding proteins that play diverse roles in mRNA
processing and regulation. The founding member, Drosophila Elav, was recognized as a …

Although splicing occurs largely co-transcriptionally, the order by which introns are removed
does not necessarily follow the order in which they are transcribed. Whereas several …

As a highly prevalent neurodevelopmental disorder, attention-deficit hyperactivity disorder
(ADHD) mostly affects children and adolescents across the globe, and it is regarded as a …

Motivation It is necessary to develop exploratory tools to learn from the unprecedented
volume of high-dimensional multi-omic data currently being produced. We have developed …

Opioid use disorder (OUD) is a national and global public health crisis with no end in sight.
While studies from animal models hint at widespread epigenetic and transcriptomic …

Psychiatric diseases are a major public health burden worldwide, yet little is known about
their underlying molecular mechanisms. Among those highly polygenic diseases …

With the recent advancements in genome editing, next generation sequencing (NGS), and
scalable cloning techniques, scientists can now conduct genetic screens at unprecedented …

Neuronal differentiation is a complex process that entails extensive morphological,
transcriptional, metabolic, and functional changes that dictate neuronal lineage commitment …