PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings

S Yang, RE Rothman - The Lancet infectious diseases, 2004 - thelancet.com
Molecular diagnostics are revolutionising the clinical practice of infectious disease. Their
effects will be significant in acute-care settings where timely and accurate diagnostic tools …

Point-of-care diagnostics for global health

P Yager, GJ Domingo, J Gerdes - Annu. Rev. Biomed. Eng., 2008 - annualreviews.org
Biomedical engineers have traditionally developed technologies in response to the needs of
the developed world's medical community. As a result, the diagnostic systems on which they …

Macrolide resistance in Campylobacter jejuni and Campylobacter coli

A Gibreel, DE Taylor - Journal of Antimicrobial Chemotherapy, 2006 - academic.oup.com
Infection with Campylobacter jejuni is now considered to be the most common cause of
acute bacterial gastroenteritis in humans worldwide. It occurs more frequently than infections …

Exploiting molecular methods to explore endodontic infections: part 1—current molecular technologies for microbiological diagnosis

JF Siqueira Jr, IN Rôças - Journal of endodontics, 2005 - Elsevier
Endodontic infections have been traditionally studied by culture-dependent methods.
However, as with other areas of clinical microbiology, culture-based investigations are …

Mechanisms of antimicrobial resistance: their clinical relevance in the new millennium

AM Sefton - Drugs, 2002 - Springer
Antimicrobials show selective toxicity. Suitable targets for antimicrobials to act at include the
bacterial cell wall, bacterial protein and folic acid synthesis, nucleic acid metabolism in …

PCR methodology as a valuable tool for identification of endodontic pathogens

JF Siqueira Jr, IN Rôças - Journal of dentistry, 2003 - Elsevier
Objectives: This paper reviews the principles of polymerase chain reaction (PCR)
methodology, its application in identification of endodontic pathogens and the perspectives …

Molecular genetic methods for the diagnosis of fastidious microorganisms

F Fenollar, D Raoult - Apmis, 2004 - Wiley Online Library
Technological innovations in the detection and identification of microorganisms using
molecular techniques such as polymerase chain reaction (PCR) have ushered in a new era …

Microbiological evaluation of acute periradicular abscesses by DNA-DNA hybridization

JF Siqueira Jr, IN Rôças, R Souto, M de Uzeda… - Oral Surgery, Oral …, 2001 - Elsevier
Objective: The purpose of the present investigation was to examine the microbiota of acute
periradicular abscesses of endodontic origin by using a molecular genetic method. Study …

An integrated microfluidic platform for sensitive and rapid detection of biological toxins

RJ Meagher, AV Hatch, RF Renzi, AK Singh - Lab on a Chip, 2008 - pubs.rsc.org
Towards designing a portable diagnostic device for detecting biological toxins in bodily
fluids, we have developed microfluidic chip-based immunoassays that are rapid (< 20 …

A rapid DNA biosensor for the molecular diagnosis of infectious disease

AL Chua, CY Yean, M Ravichandran, BH Lim… - Biosensors and …, 2011 - Elsevier
Treating patients with infectious diseases relies heavily on rapid and proper diagnosis.
Molecular detection such as PCR has become increasingly important and efforts have been …