[HTML][HTML] Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications

B Wiltschi, T Cernava, A Dennig, MG Casas… - Biotechnology …, 2020 - Elsevier
Competitive sustainable production in industry demands new and better biocatalysts,
optimized bioprocesses and cost-effective product recovery. Our review sheds light on the …

Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry

MM Gomari, N Saraygord-Afshari, M Farsimadan… - Biotechnology …, 2020 - Elsevier
Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

[HTML][HTML] The E. coli pET expression system revisited—mechanistic correlation between glucose and lactose uptake

DJ Wurm, L Veiter, S Ulonska, B Eggenreich… - Applied microbiology …, 2016 - Springer
Therapeutic monoclonal antibodies are mainly produced in mammalian cells to date.
However, unglycosylated antibody fragments can also be produced in the bacterium …

[HTML][HTML] Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis

AM Erian, M Gibisch, S Pflügl - Microbial Cell Factories, 2018 - Springer
Background Efficient microbial production of chemicals is often hindered by the cytotoxicity
of the products or by the pathogenicity of the host strains. Hence 2, 3-butanediol, an …

Towards biobased industry: acetate as a promising feedstock to enhance the potential of microbial cell factories

K Novak, S Pflügl - FEMS microbiology letters, 2018 - academic.oup.com
A broad range of different chemical and pharmaceutical compounds have been produced in
microbial cell factories. To compete with traditional crude oil based production processes …

[HTML][HTML] Strategic optimization of conditions for the solubilization of GST-tagged amphipathic helix-containing ciliary proteins overexpressed as inclusion bodies in E …

AA Shendge, JS D'Souza - Microbial cell factories, 2022 - Springer
Expression of affinity-tagged recombinant proteins for crystallography, protein–protein
interaction, antibody generation, therapeutic applications, etc. mandates the generation of …

Surface biofunctionalization to improve the efficacy of biomaterial substrates to be used in regenerative medicine

MR Casanova, RL Reis, A Martins, NM Neves - Materials Horizons, 2020 - pubs.rsc.org
Surface biofunctionalization has emerged in the past decade as a rising tool in Tissue
Engineering and Regenerative Medicine (TERM) approaches. The development of …

Controlled Non‐Viral Gene Delivery in Cartilage and Bone Repair: Current Strategies and Future Directions

T Gonzalez‐Fernandez, DJ Kelly… - Advanced …, 2018 - Wiley Online Library
Recent advances in the approval and commercialization of gene therapeutics have fostered
the return of gene therapy to center stage. But despite new optimism, no Food and Drug …

[HTML][HTML] Characterizing the effect of expression of an acetyl-CoA synthetase insensitive to acetylation on co-utilization of glucose and acetate in batch and continuous …

K Novak, L Flöckner, AM Erian, P Freitag… - Microbial Cell …, 2018 - Springer
Background Due to its high stress tolerance and low acetate secretion, Escherichia coli W is
reported to be a good production host for several metabolites and recombinant proteins …

Organophosphate-hydrolyzing enzymes as first-line of defence against nerve agent-poisoning: Perspectives and the road ahead

ARS Iyengar, AH Pande - The protein journal, 2016 - Springer
Nerve agents (NAs) are extremely neurotoxic synthetic organophosphate (OP) compounds
exploited as weapons of mass destruction in terrorist attacks and chemical warfare …