Cancer is a complex disease resulting from the genetic and epigenetic disruption of normal
cells. The mechanistic understanding of the pathways involved in tumor transformation has …

▪ Abstract Large-genome eukaryotes use heritable cytosine methylation to silence
promoters, especially those associated with transposons and imprinted genes. Cytosine …

Motivation: DNA methylation is an epigenetic mechanism of gene regulation. Bisulfite-
conversion-based PCR methods, such as bisulfite sequencing PCR (BSP) and methylation …

Defects in apoptosis underpin both tumorigenesis and drug resistance, and because of
these defects chemotherapy often fails. Understanding the molecular events that contribute …

Betaine is an essential osmolyte and source of methyl groups and comes from either the diet
or by the oxidation of choline. Its metabolism methylates homocysteine to methionine, also …

Multiplex Ligation-dependent Probe Amplification (MLPA) assay is a recently developed
technique able to evidence variations in the copy number of several human genes. Due to …

Despite the increased interest in epigenetic research, its progress has been hampered by a
lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now …

Recent studies suggest that assisted reproductive technologies (ART), which involve the
isolation, handling and culture of gametes and early embryos, are associated with an …

DNA methylation is a major epigenetic factor that has been postulated to regulate cell
lineage differentiation. We report here that conditional gene deletion of the maintenance …

Imprinted genes are differentially marked during germ cell development to allow for their
eventual parent-of-origin specific expression. A subset of imprinted genes becomes …