Metagenomics: novel enzymes from non-culturable microbes

F Berini, C Casciello, GL Marcone… - FEMS microbiology …, 2017 - academic.oup.com
In the transition to the post-petroleum economy, there is a growing demand for novel
enzymes with high process performances to replace traditional chemistry with a more …

β-Xylosidases: Structural diversity, catalytic mechanism, and inhibition by monosaccharides

A Rohman, BW Dijkstra, NNT Puspaningsih - International Journal of …, 2019 - mdpi.com
Xylan, a prominent component of cellulosic biomass, has a high potential for degradation
into reducing sugars, and subsequent conversion into bioethanol. This process requires a …

Metagenomic screening strategies for bioprospecting enzymes from environmental samples

AK Wani, F Rahayu, FT Kadarwati… - … Series: Earth and …, 2022 - iopscience.iop.org
Globally, there is a growing demand for biocatalysts because of the associated efficacy and
efficiency. The applications of enzymes in food, paper, pulp, textile, and chemical industries …

Cellulases from thermophiles found by metagenomics

JJ Escuder-Rodríguez, ME DeCastro, ME Cerdán… - Microorganisms, 2018 - mdpi.com
Cellulases are a heterogeneous group of enzymes that synergistically catalyze the
hydrolysis of cellulose, the major component of plant biomass. Such reaction has …

Improving the catalytic activity of β-glucosidase from Coniophora puteana via semi-rational design for efficient biomass cellulose degradation

HY Zhou, Q Chen, YF Zhang, DD Chen, XN Yi… - Enzyme and Microbial …, 2023 - Elsevier
In order to improve the degradation activity of β-glucosidase (CpBgl) from Coniophora
puteana, the structural modification was conducted. The enzyme activity of mutants CpBgl …

Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences

J Cheng, T Romantsov, K Engel, AC Doxey, DR Rose… - PLoS …, 2017 - journals.plos.org
The techniques of metagenomics have allowed researchers to access the genomic potential
of uncultivated microbes, but there remain significant barriers to determination of gene …

Benchmarking of density functionals for the kinetics and thermodynamics of the hydrolysis of glycosidic bonds catalyzed by glycosidases

AT Pereira, AJM Ribeiro, PA Fernandes… - … Journal of Quantum …, 2017 - Wiley Online Library
In recent years, there has been an increased interest in understanding the enzymatic
mechanism of glycosidases resorting mostly to DFT and DFT/MM calculations. However, the …

Crystal structure and identification of amino acid residues for catalysis and binding of GH3 AnBX β-xylosidase from Aspergillus niger

W Kaenying, K Choengpanya, T Tagami… - Applied Microbiology …, 2023 - Springer
Abstract β-Xylosidases catalyze the hydrolysis of xylooligosaccharides to xylose in the final
step of hemicellulose degradation. AnBX, which is a GH3 β-xylosidase from Aspergillus …

Novel β-Glucosidase Mibgl3 from Microbacterium sp. XT11 with Oligoxanthan-Hydrolyzing Activity

J Gu, D Wang, Q Wang, W Liu, X Chen… - Journal of Agricultural …, 2022 - ACS Publications
The enzymatic pathway of xanthan depolymerization has been predicted previously;
however, the β-glucosidase and unsaturated glucuronyl hydrolase in this system have not …

Metagenomics in the search for industrial enzymes

T Ahmad, RS Singh, G Gupta, A Sharma… - Advances in Enzyme …, 2019 - Elsevier
From bacteria to man, all living organisms are built and maintained by biocatalysts called
enzymes. Microorganisms are preferential sources for the production of industrial enzymes …