Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …

The global leather industry has been at the receiving end of various environmental and
ethical backlash as it mainly relies on animal agriculture which contributes to deforestation …

Filamentous fungi are competitive hosts for the production of drugs, proteins, and chemicals.
However, their utility is limited by screening methods and low throughput. In this work, a …

丝状真菌能够合成抗生素, 色素, 酶制剂, 激素等多种天然产物, 广泛应用于医药, 化工,
农业和基础生物学研究等领域. 丝状真菌遗传背景复杂, 阻碍了对其的进一步开发利用 …

Natural products are a rich resource for the discovery of innovative drugs. Microbial
cocultivation enables discovery of novel natural products through tandem enzymatic …

Using CRISPR-Cas9 technology and a microhomology-mediated end-joining repair system,
we substituted genes of the gliotoxin pathway in Aspergillus fumigatus with genes …

Commonly used fungal transformation protocols rely on the use of either electroporation or
the lithium acetate/single strand carrier DNA/Polyethylene glycol/heat shock method. We …

The genus Fusarium is well-known to comprise many pathogenic fungi that affect cereal
crops worldwide, causing severe damage to agriculture and the economy. In this study, an …

Episomal AMA1-based plasmids are increasingly used for expressing biosynthetic pathways
and CRISPR/Cas systems in filamentous fungi cell factories due to their high transformation …