The expanded CRISPR toolbox for constructing microbial cell factories
Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …
[HTML][HTML] Fungal mycelium as leather alternative: A sustainable biogenic material for the fashion industry
The global leather industry has been at the receiving end of various environmental and
ethical backlash as it mainly relies on animal agriculture which contributes to deforestation …
ethical backlash as it mainly relies on animal agriculture which contributes to deforestation …
Core–Shell Droplet-Based Microfluidic Screening System for Filamentous Fungi
C Zhang, X Wu, F Song, S Liu, S Yu, J Zhou - ACS sensors, 2023 - ACS Publications
Filamentous fungi are competitive hosts for the production of drugs, proteins, and chemicals.
However, their utility is limited by screening methods and low throughput. In this work, a …
However, their utility is limited by screening methods and low throughput. In this work, a …
CRISPR/Cas 基因组编辑技术在丝状真菌次级代谢产物合成中的应用
林继聪, 邹根, 刘宏民, 魏勇军 - 合成生物学, 2023 - synbioj.cip.com.cn
丝状真菌能够合成抗生素, 色素, 酶制剂, 激素等多种天然产物, 广泛应用于医药, 化工,
农业和基础生物学研究等领域. 丝状真菌遗传背景复杂, 阻碍了对其的进一步开发利用 …
农业和基础生物学研究等领域. 丝状真菌遗传背景复杂, 阻碍了对其的进一步开发利用 …
Monaspin B, a Novel Cyclohexyl-furan from Cocultivation of Monascus purpureus and Aspergillus oryzae, Exhibits Potent Antileukemic Activity
Z Meng, Y Tan, Y Duan, M Li - Journal of Agricultural and Food …, 2024 - ACS Publications
Natural products are a rich resource for the discovery of innovative drugs. Microbial
cocultivation enables discovery of novel natural products through tandem enzymatic …
cocultivation enables discovery of novel natural products through tandem enzymatic …
Reconstitution of the Early Stage of Chetomin Biosynthesis in Aspergillus fumigatus Leads to the Production of Epipolythiodioxopiperazines
Y Guo, JZ Liu, J Limwachiranon, F Xu, Y Han… - Organic …, 2024 - ACS Publications
Using CRISPR-Cas9 technology and a microhomology-mediated end-joining repair system,
we substituted genes of the gliotoxin pathway in Aspergillus fumigatus with genes …
we substituted genes of the gliotoxin pathway in Aspergillus fumigatus with genes …
Improved gene-targeting efficiency upon starvation in Saccharomycopsis
D Kaimenyi, M Rij, J Wendland - Fungal Genetics and Biology, 2023 - Elsevier
Commonly used fungal transformation protocols rely on the use of either electroporation or
the lithium acetate/single strand carrier DNA/Polyethylene glycol/heat shock method. We …
the lithium acetate/single strand carrier DNA/Polyethylene glycol/heat shock method. We …
[HTML][HTML] Genomic and Metabolomic Analysis of the Endophytic Fungus Fusarium sp. VM-40 Isolated from the Medicinal Plant Vinca minor
T He, X Li, R Iacovelli, T Hackl, K Haslinger - Journal of Fungi, 2023 - mdpi.com
The genus Fusarium is well-known to comprise many pathogenic fungi that affect cereal
crops worldwide, causing severe damage to agriculture and the economy. In this study, an …
crops worldwide, causing severe damage to agriculture and the economy. In this study, an …
Next generation AMA1-based plasmids for enhanced heterologous expression in filamentous fungi
Episomal AMA1-based plasmids are increasingly used for expressing biosynthetic pathways
and CRISPR/Cas systems in filamentous fungi cell factories due to their high transformation …
and CRISPR/Cas systems in filamentous fungi cell factories due to their high transformation …