Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Unique, functional, homodimeric heavy chain–only antibodies, devoid of light chains, are
circulating in the blood of Camelidae. These antibodies recognize their cognate antigen via …

Specialized epitope tags are widely used for detecting, manipulating or purifying proteins,
but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Advances in super-resolution microscopy have demonstrated single-molecule localization
precisions of a few nanometers. However, translation of such high localization precisions …

Nanobodies are the recombinant variable domains of heavy-chain-only antibodies, with
many unique properties such as small size, excellent solubility, superior stability, quick …

The brain is arguably the most complex organ. The branched and extended morphology of
nerve cells, their subcellular complexity, the multiplicity of brain cell types as well as their …

Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically
expanded biological samples with conventional microscopes. By combining ExM with single …

The targeted delivery of chemotherapeutic drugs is a major challenge in the clinical
treatment of cancer. Herein, we constructed a multifunctional DNA nanoplatform as a …

To expand the toolbox of imaging in living cells, we have engineered a single-chain variable
fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting …