[PDF][PDF] Cultureof pig embryos

RM Petters, KD Wells - Journal of Reproduction and Fertility …, 1993 - researchgate.net
Pig embryos can be cultured using a number of different strategies including complex
approaches like culture in vivo in a surrogate oviduct (rabbit, sheep, mouse), culture in …

Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro

CF Rosenkrans Jr, NL First - Journal of animal science, 1994 - academic.oup.com
Due to the complicated media used for culturing bovine embryos, most of the nutrient
requirements are unknown. Recently, we developed a simple, serum-free medium (CR1) …

In vitro culture of sheep embryos without co-culture: successes and perspectives

SK Walker, TM Heard, RF Seamark - Theriogenology, 1992 - Elsevier
The finding that zygotes (one-cell embryos) from several livestock species can be routinely
cultured in relatively simple media to the blastocyst stage questions the nature of the …

Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro

RM Petters, BH Johnson, ML Reed… - …, 1990 - rep.bioscientifica.com
Pig embryos at the 1-or 2-cell stage (before the'block'to development in vitro) were cultured
in 8 different media derived from Krebs'–Ringer–bicarbonate medium. A 2× 2× 2 factorial …

Introduction of a porcine growth hormone fusion gene into transgenic pigs promotes growth

PD Vize, AE Michalska, R Ashman… - Journal of Cell …, 1988 - journals.biologists.com
Six transgenic pigs have been produced by microinjecting a human metallothionein
promoter/porcine growth hormone gene construct into the pronuclei of fertilized eggs which …

Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro

JE Swain, CL Bormann, SG Clark, EM Walters… - …, 2002 - rep.bioscientifica.com
The effects of four reagents on the activation and subsequent fertilization of equine oocytes,
and the development of these after intracytoplasmic sperm injection, were investigated …

[PDF][PDF] Effects of oxygen, CO2/pH and medium on the in vitro development of individually cultured porcine one-and two-cell embryos

F Berthelot, M Terqui - Reproduction Nutrition Development, 1996 - rnd.edpsciences.org
Summary ― The gas atmosphere and medium composition are critical factors in the
in vitro development of one-and two-cell embryos of several species. The present study …

Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1. 2/G2. 2 sequential medium

JE Swain, CL Bormann, RL Krisher - Theriogenology, 2001 - Elsevier
Porcine embryo development in vitro is relatively inefficient compared to other domestic
species. Currently, a single culture medium (NCSU23) is the standard for porcine in vitro …

Development of in vitro fertilized feline embryos in a modified Earle's balanced salt solution: influence of protein supplements and culture dishes on fertilization …

M Kanda, T MIYAZAKI, M KANDA, H NAKAO… - Journal of veterinary …, 1998 - jstage.jst.go.jp
The effects of protein supplements and culture dish type on in vitro fertilization (IVF) and
embryo development in culture were examined in the domestic cat. In Experiment I, follicular …

Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro

DB Koo, NH Kim, HT Lee, KS Chung - Theriogenology, 1997 - Elsevier
The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …