Mammalian cell expression has become the dominant recombinant protein production
system for clinical applications because of its capacity for post-translational modification and …

In the biopharmaceutical industry, mammalian cell culture systems, especially Chinese
hamster ovary (CHO) cells, are predominantly used for the production of therapeutic …

Cell-free systems (CFS) have recently evolved into key platforms for synthetic biology
applications. Many synthetic biology tools have traditionally relied on cell-based systems …

Glycoproteins generally exist as populations of glycosylated variants (glycoforms) of a single
polypeptide. Although the same glycosylation machinery is available to all proteins that …

Glycosylation is the most extensive of all the posttranslational modifications, and has
important functions in the secretion, antigenicity and clearance of glycoproteins. In recent …

Recent technical advances have revitalized cell-free expression systems to meet the
increasing demands for protein synthesis. Cell-free systems offer several advantages over …

The Campylobacter jejuni pgl locus encodes an N‐linked protein glycosylation machinery
that can be functionally transferred into Escherichia coli. In this system, we analyzed the …

The biophysical rules that govern folding of small, single‐domain proteins in dilute solutions
are now quite well understood. The mechanisms underlying co‐translational folding of …

Many eukaryotic proteins are modified by Asn-linked (N-linked) glycosylation. The number
and position of oligosaccharides added to a protein by the enzyme …

N-Linked glycosylation is a common form of protein processing that can profoundly affect
protein expression, structure, and function. N-Linked glycosylation generally occurs at the …