[HTML][HTML] Current concepts, advances, and challenges in deciphering the human microbiota with metatranscriptomics

T Ojala, AE Häkkinen, E Kankuri, M Kankainen - Trends in Genetics, 2023 - cell.com
Metatranscriptomics refers to the analysis of the collective microbial transcriptome of a
sample. Its increased utilization for the characterization of human-associated microbial …

Integrated “lab-on-a-chip” microfluidic systems for isolation, enrichment, and analysis of cancer biomarkers

S Surappa, P Multani, U Parlatan, PD Sinawang… - Lab on a Chip, 2023 - pubs.rsc.org
The liquid biopsy has garnered considerable attention as a complementary clinical tool for
the early detection, molecular characterization and monitoring of cancer over the past …

Unlocking the efficiency of genomics laboratories with robotic liquid-handling

H Tegally, JE San, J Giandhari, T de Oliveira - BMC genomics, 2020 - Springer
In research and clinical genomics laboratories today, sample preparation is the bottleneck of
experiments, particularly when it comes to high-throughput next generation sequencing …

rRT-PCR for SARS-CoV-2: Analytical considerations

R Rahbari, N Moradi, M Abdi - Clinica Chimica Acta, 2021 - Elsevier
The COVID-19 pandemic remains a significant problem involving health systems worldwide.
Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS …

Nucleic acid extraction: Fundamentals of sample preparation methodologies, current advancements, and future endeavors

MN Emaus, M Varona, DR Eitzmann, SA Hsieh… - TrAC Trends in …, 2020 - Elsevier
The extraction of nucleic acids (NA) is an essential component of NA analysis. Without
sufficient extraction and purification, contaminants co-existing with NAs often inhibit …

[PDF][PDF] The evolution of DNA extraction methods

M Dairawan, PJ Shetty - Am. J. Biomed. Sci. Res, 2020 - pdfs.semanticscholar.org
Since the first DNA extraction performed by Friedrich Miescher in 1869, scientists have
made extraordinary progress in designing extraction methods that are more reliable, easier …

Advancements in CRISPR-based biosensing for next-gen point of care diagnostic application

A Kumaran, N Jude Serpes, T Gupta, A James… - Biosensors, 2023 - mdpi.com
With the move of molecular tests from diagnostic labs to on-site testing becoming more
common, there is a sudden rise in demand for nucleic acid-based diagnostic tools that are …

Extraction of viral nucleic acids with carbon nanotubes increases SARS-CoV-2 quantitative reverse transcription polymerase chain reaction detection sensitivity

S Jeong, E González-Grandío, N Navarro, RL Pinals… - ACS …, 2021 - ACS Publications
The global SARS-CoV-2 coronavirus pandemic has led to a surging demand for rapid and
efficient viral infection diagnostic tests, generating a supply shortage in diagnostic test …

Challenges and opportunities for clustered regularly interspaced short palindromic repeats based molecular biosensing

M Bao, Q Chen, Z Xu, EC Jensen, C Liu, JT Waitkus… - ACS …, 2021 - ACS Publications
Clustered regularly interspaced short palindromic repeats, CRISPR, has recently emerged
as a powerful molecular biosensing tool for nucleic acids and other biomarkers due to its …

Combining sensors and actuators with electrowetting-on-dielectric (EWOD): advanced digital microfluidic systems for biomedical applications

Z Tong, C Shen, Q Li, H Yin, H Mao - Analyst, 2023 - pubs.rsc.org
The concept of digital microfluidics (DMF) enables highly flexible and precise droplet
manipulation at a picoliter scale, making DMF a promising approach to realize integrated …