[HTML][HTML] Progression of LAMP as a result of the COVID-19 pandemic: is PCR finally rivaled?

C Mannier, JY Yoon - Biosensors, 2022 - mdpi.com
Reflecting on the past three years and the coronavirus disease 19 (COVID-19) pandemic,
varying global tactics offer insights into the most effective public-health responses. In the US …

Multiplexed centrifugal microfluidic system for dynamic solid-phase purification of polynucleic acids direct from buccal swabs

LM Dignan, MS Woolf, CJ Tomley… - Analytical …, 2021 - ACS Publications
This report describes the development of a centrifugally controlled microfluidic dynamic solid-
phase extraction (dSPE) platform to reliably obtain amplification-ready nucleic acids (NAs) …

DNA binding to the silica: cooperative adsorption in action

S Bag, S Rauwolf, SP Schwaminger, W Wenzel… - Langmuir, 2021 - ACS Publications
The adsorption and desorption of nucleic acid to a solid surface is ubiquitous in various
research areas like pharmaceutics, nanotechnology, molecular biology, and molecular …

[HTML][HTML] Point-of-care or point-of-need diagnostic tests: time to change outbreak investigation and pathogen detection

S Hansen, A Abd El Wahed - Tropical Medicine and Infectious Disease, 2020 - mdpi.com
In the recent years, the progress of international trade and travel has led to an increased risk
of emerging infections. Around 75 percent of the pathogens causing these infections are of …

Fabrication of a foldable all-in-one point-of-care molecular diagnostic microdevice for the facile identification of multiple pathogens

KTL Trinh, RA Stabler, NY Lee - Sensors and Actuators B: Chemical, 2020 - Elsevier
In the present study, we fabricated a foldable all-in-one microdevice for the nucleic acid test
(NAT) performing DNA extraction, polymerase chain reaction (PCR), and on-site colorimetric …

DNA extraction and host depletion methods significantly impact and potentially bias bacterial detection in a biological fluid

E Ganda, KL Beck, N Haiminen, JD Silverman… - Msystems, 2021 - Am Soc Microbiol
Untargeted sequencing of nucleic acids present in food can inform the detection of food
safety and origin, as well as product tampering and mislabeling issues. The application of …

The application of circulating tumor cell and cell-free DNA liquid biopsies in ovarian cancer

A Balla, J Bhak, O Biró - Molecular and Cellular Probes, 2022 - Elsevier
Ovarian cancer is the deadliest gynecological cancer. 70% of the cases are diagnosed at
late stages with already developed metastases due to the absence of easily noticeable …

A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening

K Tsougeni, AS Kastania, GD Kaprou, M Eck… - Sensors and Actuators B …, 2019 - Elsevier
Strict regulations govern food safety as the latter is of extremely high importance and a major
public issue, while it is demanding in respect of analytical effort. The duration of common …

Isolation of high-molecular-weight DNA for long-read sequencing using a high-salt gel Electroelution trap

R Kalendar, KI Ivanov, O Samuilova, U Kairov… - Analytical …, 2023 - ACS Publications
Long-read sequencing technologies require high-molecular-weight (HMW) DNA of sufficient
purity and integrity, which can be difficult to obtain from complex biological samples. We …

Cell-free DNA: an overview of sample types and isolation procedures

F Martignano - Cell-free DNA as Diagnostic Markers: Methods and …, 2019 - Springer
The study of cell-free DNA (cfDNA) is often challenging due to genomic DNA contamination,
low concentration, and high fragmentation. Therefore, it is important to optimize pre …