Molecular detection of SARS-CoV-2 in gargle and saliva complements the standard analysis
of nasopharyngeal swabs (NPS) specimens. Although gargle and saliva specimens can be …

Nucleic acid extraction (NAE) plays a crucial role for diagnostic testing procedures. For
decades, dried blood spots (DBS) have been used for serology, drug monitoring, and …

The outbreak of COVID-19 makes epidemic prevention and control become a growing
global concern. Nucleic acid amplification testing (NAAT) can realize early and rapid …

The incidence and mortality rates of colorectal cancer (CRC) have been high in recent
years. Prevention and early detection are crucial for decreasing the death rate. Therefore …

Histopathology is the gold standard for fungal infection (FI) diagnosis, but it does not provide
a genus and/or species identification. The objective of the present study was to develop …

Polymerase chain reaction (PCR) is gold-standard method for molecular detection of
pathogens. DNA extraction from a complex biological medium is a crucial process for PCR …

The emergence of the COVID-19 pandemic prompted fast development of novel diagnostic
methods of the etiologic virus SARS-CoV-2. Methods based on CRISPR–Cas systems have …

Antimicrobial resistance (AMR) is one of today's most important concerns. Indiscriminate use
of antibiotics and their release into the water bodies have been known to afflict the bacterial …

Plasmid DNA (pDNA) isolation from bacterial cells is one of the most common and critical
steps in molecular cloning and biomedical research. Almost all pDNA purification involves …

Timely and accurate detection of the causal agent of a disease is crucial to restrict suffering
and save lives. Mere symptoms are often not enough to detect the root cause of the disease …