Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health

HK Khera, R Mishra - Molecular Biotechnology, 2023 - Springer
Timely and accurate detection of the causal agent of a disease is crucial to restrict suffering
and save lives. Mere symptoms are often not enough to detect the root cause of the disease …

Understanding quantitative polymerase chain reaction bioanalysis issues before validation planning: Japan Bioanalysis Forum discussion group

A Uchiyama, Y Naritomi, Y Hashimoto, T Hanada… - Bioanalysis, 2022 - Taylor & Francis
Investigating the biodistribution of cell and gene therapy products may play an important role
in evaluating their safety and pharmacology. As quantitative polymerase chain reaction …

Tool and techniques study to plant microbiome current understanding and future needs: an overview

Enespa, P Chandra - Communicative & Integrative Biology, 2022 - Taylor & Francis
Microorganisms are present in the universe and they play role in beneficial and harmful to
human life, society, and environments. Plant microbiome is a broad term in which microbes …

[HTML][HTML] Enzymatic and chemical-based methods to inactivate endogenous blood ribonucleases for nucleic acid diagnostics

AT Bender, BP Sullivan, L Lillis, JD Posner - The Journal of Molecular …, 2020 - Elsevier
There are ongoing research efforts into simple and low-cost point-of-care nucleic acid
amplification tests (NATs) addressing widespread diagnostic needs in resource-limited …

[HTML][HTML] Engineered yeast genomes accurately assembled from pure and mixed samples

JH Collins, KW Keating, TR Jones, S Balaji… - Nature …, 2021 - nature.com
Yeast whole genome sequencing (WGS) lacks end-to-end workflows that identify genetic
engineering. Here we present Prymetime, a tool that assembles yeast plasmids and …

Advances in point-of-care genetic testing for personalized medicine applications

AS De Olazarra, SX Wang - Biomicrofluidics, 2023 - pubs.aip.org
Breakthroughs within the fields of genomics and bioinformatics have enabled the
identification of numerous genetic biomarkers that reflect an individual's disease …

Uracil-DNA-glycosylase-assisted loop-mediated isothermal amplification for detection of bacteria from urine samples with reduced contamination

Y Zeng, M Liu, Y Xia, X Jiang - Analyst, 2020 - pubs.rsc.org
Loop-mediated isothermal amplification (LAMP) is a useful molecular biology technology for
analytical applications, but it is prone to contamination because of escaped aerosols …

[HTML][HTML] Isolation of nucleic acids using liquid–liquid phase separation of pH-sensitive elastin-like polypeptides

T Díez Pérez, AN Tafoya, DS Peabody, MR Lakin… - Scientific Reports, 2024 - nature.com
Extraction of nucleic acids (NAs) is critical for many methods in molecular biology and
bioanalytical chemistry. NA extraction has been extensively studied and optimized for a wide …

[HTML][HTML] mRNA extraction from lipid nanoparticles

C Malburet, A Carboni, S Guinamand, H Naik… - … of Chromatography A, 2024 - Elsevier
Abstract Messenger RiboNucleic Acid (mRNA) vaccines have recently shown considerable
promises for both prophylactic and therapeutic vaccines. These vaccines do not carry an …

An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detection

LM Dignan, R Turiello, TR Layne, KC O'Connell… - Analytica Chimica …, 2021 - Elsevier
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic RNA virus
characterized by high transmission rates and pathogenicity worldwide. Continued control of …