Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …

A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

Investigations of CRISPR gene knockout editing profiles have contributed to enhanced
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …

Astrocytes become reactive in response to insults to the central nervous system by adopting
context-specific cellular signatures and outputs, but a systematic understanding of the …

Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics
involves introducing biomolecular cargoes into cells. However, successful delivery is a …

Efficient precision genome engineering requires high frequency and specificity of integration
at the genomic target site. Here, we describe a set of resources to streamline reporter gene …

Labeling stem cells with magnetic nanoparticles is a promising technique for in vivo tracking
and magnetic targeting of transplanted stem cells, which is critical for improving the …

Background Primary Sjogren's syndrome (pSS) is a slowly progressive, inflammatory
autoimmune disease characterized by lymphocytic infiltration into salivary and lacrimal …

Human pluripotent stem cells (hPSCs) are capable of extensive self-renewal yet remain
highly sensitive to environmental perturbations in vitro, posing challenges to their …