Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Natural variation in crops: realized understanding, continuing promise

Y Liang, HJ Liu, J Yan, F Tian - Annual review of plant biology, 2021 - annualreviews.org
Crops feed the world's population and shape human civilization. The improvement of crop
productivity has been ongoing for almost 10,000 years and has evolved from an experience …

A graph-based genome and pan-genome variation of the model plant Setaria

Q He, S Tang, H Zhi, J Chen, J Zhang, H Liang… - Nature …, 2023 - nature.com
Setaria italica (foxtail millet), a founder crop of East Asian agriculture, is a model plant for C4
photosynthesis and developing approaches to adaptive breeding across multiple climates …

Pan-genome analysis of 33 genetically diverse rice accessions reveals hidden genomic variations

P Qin, H Lu, H Du, H Wang, W Chen, Z Chen, Q He… - Cell, 2021 - cell.com
Structural variations (SVs) and gene copy number variations (gCNVs) have contributed to
crop evolution, domestication, and improvement. Here, we assembled 31 high-quality …

[HTML][HTML] Pan-genome of wild and cultivated soybeans

Y Liu, H Du, P Li, Y Shen, H Peng, S Liu, GA Zhou… - Cell, 2020 - cell.com
Soybean is one of the most important vegetable oil and protein feed crops. To capture the
entire genomic diversity, it is needed to construct a complete high-quality pan-genome from …

Major impacts of widespread structural variation on gene expression and crop improvement in tomato

M Alonge, X Wang, M Benoit, S Soyk, L Pereira… - Cell, 2020 - cell.com
Structural variants (SVs) underlie important crop improvement and domestication traits.
However, resolving the extent, diversity, and quantitative impact of SVs has been …

Eight high-quality genomes reveal pan-genome architecture and ecotype differentiation of Brassica napus

JM Song, Z Guan, J Hu, C Guo, Z Yang, S Wang, D Liu… - Nature plants, 2020 - nature.com
Rapeseed (Brassica napus) is the second most important oilseed crop in the world but the
genetic diversity underlying its massive phenotypic variations remains largely unexplored …

Cotton pan-genome retrieves the lost sequences and genes during domestication and selection

J Li, D Yuan, P Wang, Q Wang, M Sun, Z Liu, H Si, Z Xu… - Genome biology, 2021 - Springer
Background Millennia of directional human selection has reshaped the genomic architecture
of cultivated cotton relative to wild counterparts, but we have limited understanding of the …

Impacts of allopolyploidization and structural variation on intraspecific diversification in Brassica rapa

X Cai, L Chang, T Zhang, H Chen, L Zhang, R Lin… - Genome biology, 2021 - Springer
Background Despite the prevalence and recurrence of polyploidization in the speciation of
flowering plants, its impacts on crop intraspecific genome diversification are largely …

Pan-genome analysis of 13 Malus accessions reveals structural and sequence variations associated with fruit traits

T Wang, S Duan, C Xu, Y Wang, X Zhang, X Xu… - Nature …, 2023 - nature.com
Structural variations (SVs) and copy number variations (CNVs) contribute to trait variations in
fleshy-fruited species. Here, we assemble 10 genomes of genetically diverse Malus …