Uncovering the complexity of transcriptomes with RNA‐Seq
In recent years, the introduction of massively parallel sequencing platforms for Next
Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred …
Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred …
High‐throughput sequencing for biology and medicine
WW Soon, M Hariharan, MP Snyder - Molecular systems biology, 2013 - embopress.org
Advances in genome sequencing have progressed at a rapid pace, with increased
throughput accompanied by plunging costs. But these advances go far beyond faster and …
throughput accompanied by plunging costs. But these advances go far beyond faster and …
Removing technical variability in RNA-seq data using conditional quantile normalization
The ability to measure gene expression on a genome-wide scale is one of the most
promising accomplishments in molecular biology. Microarrays, the technology that first …
promising accomplishments in molecular biology. Microarrays, the technology that first …
A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case …
I Nookaew, M Papini, N Pornputtapong… - Nucleic acids …, 2012 - academic.oup.com
RNA-seq, has recently become an attractive method of choice in the studies of
transcriptomes, promising several advantages compared with microarrays. In this study, we …
transcriptomes, promising several advantages compared with microarrays. In this study, we …
[HTML][HTML] Large scale comparison of gene expression levels by microarrays and RNAseq using TCGA data
RNAseq and microarray methods are frequently used to measure gene expression level.
While similar in purpose, there are fundamental differences between the two technologies …
While similar in purpose, there are fundamental differences between the two technologies …
[HTML][HTML] A high-throughput method for Illumina RNA-Seq library preparation
R Kumar, Y Ichihashi, S Kimura, DH Chitwood… - Frontiers in plant …, 2012 - frontiersin.org
With the introduction of cost effective, rapid, and superior quality next generation sequencing
techniques, gene expression analysis has become viable for labs conducting small projects …
techniques, gene expression analysis has become viable for labs conducting small projects …
Identification and Properties of 1,119 Candidate LincRNA Loci in the Drosophila melanogaster Genome
RS Young, AC Marques, C Tibbit… - Genome biology and …, 2012 - academic.oup.com
The functional repertoire of long intergenic noncoding RNA (lincRNA) molecules has begun
to be elucidated in mammals. Determining the biological relevance and potential gene …
to be elucidated in mammals. Determining the biological relevance and potential gene …
RNA-Seq analysis and de novo transcriptome assembly of Hevea brasiliensis
Z Xia, H Xu, J Zhai, D Li, H Luo, C He, X Huang - Plant molecular biology, 2011 - Springer
Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely
economically important crop. In an effort to facilitate biological, biochemical and molecular …
economically important crop. In an effort to facilitate biological, biochemical and molecular …
Simultaneous isolation of high-quality DNA, RNA, miRNA and proteins from tissues for genomic applications
S Peña-Llopis, J Brugarolas - Nature protocols, 2013 - nature.com
Genomic technologies have revolutionized our understanding of complex Mendelian
diseases and cancer. Solid tumors present several challenges for genomic analyses, such …
diseases and cancer. Solid tumors present several challenges for genomic analyses, such …
A strand-specific library preparation protocol for RNA sequencing
T Borodina, J Adjaye, M Sultan - Methods in enzymology, 2011 - Elsevier
The analysis of transcriptome, which was over the past decade based mostly on microarray
technologies, is now being superseded by so-called next generation sequencing (NGS) …
technologies, is now being superseded by so-called next generation sequencing (NGS) …