CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

Efficient identification of drug mechanisms of action remains a challenge. Computational
docking approaches have been widely used to predict drug binding targets; yet, such …

Antibacterial agents target the products of essential genes but rarely achieve complete
target inhibition. Thus, the all-or-none definition of essentiality afforded by traditional genetic …

Genetic tools derived from the Cas9 RNA-guided nuclease are providing essential
capabilities to study and engineer bacteria. While the importance of off-target effects was …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

Highlights•Phages need to attach to a receptor on the bacterial surface to initiate infection.•It
is not common practice to identify these receptors before phage therapy use.•Identifying …

To fully exploit the microbial genome resources, a high-throughput experimental platform is
needed to associate genes with phenotypes at the genome level. We present here a novel …

Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology
applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we …

Bacteria from the same species can differ widely in their gene content. In Escherichia coli,
the set of genes shared by all strains, known as the core genome, represents about half the …