[HTML][HTML] High-throughput mapping of the phage resistance landscape in E. coli
Bacteriophages (phages) are critical players in the dynamics and function of microbial
communities and drive processes as diverse as global biogeochemical cycles and human …
communities and drive processes as diverse as global biogeochemical cycles and human …
[HTML][HTML] Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes
L Yao, K Shabestary, SM Björk… - Nature …, 2020 - nature.com
Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology
applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we …
applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we …
[HTML][HTML] Inducible plasmid copy number control for synthetic biology in commonly used E. coli strains
The ability to externally control gene expression has been paradigm shifting for all areas of
biological research, especially for synthetic biology. Such control typically occurs at the …
biological research, especially for synthetic biology. Such control typically occurs at the …
The functional proteome landscape of Escherichia coli
Recent developments in high-throughput reverse genetics, have revolutionized our ability to
map gene function and interactions,,–. The power of these approaches depends on their …
map gene function and interactions,,–. The power of these approaches depends on their …
CRISPRi-seq for genome-wide fitness quantification in bacteria
CRISPR interference (CRISPRi) is a powerful tool to link essential and nonessential genes
to specific phenotypes and to explore their functions. Here we describe a protocol for …
to specific phenotypes and to explore their functions. Here we describe a protocol for …
Adhesive tape microfluidics with an autofocusing module that incorporates CRISPR interference: applications to long-term bacterial antibiotic studies
The ability to study bacteria at the single cell level has advanced our insights into microbial
physiology and genetics in ways not attainable by studying large populations using more …
physiology and genetics in ways not attainable by studying large populations using more …
[HTML][HTML] Gene silencing through CRISPR interference in bacteria: current advances and future prospects
R Zhang, W Xu, S Shao, Q Wang - Frontiers in Microbiology, 2021 - frontiersin.org
Functional genetic screening is an important method that has been widely used to explore
the biological processes and functional annotation of genetic elements. CRISPR/Cas …
the biological processes and functional annotation of genetic elements. CRISPR/Cas …
[HTML][HTML] Mismatch-CRISPRi reveals the co-varying expression-fitness relationships of essential genes in Escherichia coli and Bacillus subtilis
Essential genes are the hubs of cellular networks, but lack of high-throughput methods for
titrating gene expression has limited our understanding of the fitness landscapes against …
titrating gene expression has limited our understanding of the fitness landscapes against …
Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum
Acetogenic bacteria are a unique biocatalyst that highly promises to develop the sustainable
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …
bioconversion of carbon oxides (eg, CO and CO2) into multicarbon biochemicals. Genotype …
[HTML][HTML] Genome-wide promoter responses to CRISPR perturbations of regulators reveal regulatory networks in Escherichia coli
Elucidating genome-scale regulatory networks requires a comprehensive collection of gene
expression profiles, yet measuring gene expression responses for every transcription factor …
expression profiles, yet measuring gene expression responses for every transcription factor …