Enzymes maintain metabolism, and their concentration affects cellular fitness: high enzyme
levels are costly, and low enzyme levels can limit metabolic flux. Here, we used CRISPR …

CRISPR interference (CRISPRi) is the leading technique to silence gene expression in
bacteria; however, design rules remain poorly defined. We develop a best-in-class …

ABSTRACT CRISPR interference (CRISPRi) has facilitated the study of essential genes in
diverse organisms using both high-throughput and targeted approaches. Despite the …

This mini-review provides a perspective of traditional, emerging and future applications of
lactic acid bacteria (LAB) and how genome editing tools can be used to overcome current …

Bacteria with streamlined genomes, that harbor full functional genes for essential metabolic
networks, are able to synthesize the desired products more effectively and thus have …

Our bodies are colonized by a complex ecosystem of bacteria, unicellular eukaryotes and
their viruses that together play a major role in our health. Over the past few years tools …

Foodborne infection is one of the leading sources of infections spreading across the world.
Foodborne pathogens are recognized as multidrug-resistant (MDR) pathogens posing a …

Phages are one of the key ecological drivers of microbial community dynamics, function, and
evolution. Despite their importance in bacterial ecology and evolutionary processes, phage …

Genome-scale target identification promises to guide microbial cell factory engineering for
higher-titer production of biomolecules such as recombinant proteins (r-protein), but …

Highlights•Bacterial CRISPR approaches are used for gene editing, repression, and
activation.•CRISPRi, which blocks transcription of targeted genes, is the main bacterial …