The never-ending battle between lactic acid bacteria and their phages
Over the past few decades, the interest in lactic acid bacteria (LAB) has been steadily
growing. This is mainly due to their industrial use, their health benefits as probiotic bacteria …
growing. This is mainly due to their industrial use, their health benefits as probiotic bacteria …
[HTML][HTML] Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
The RNA-guided CRISPR-associated Cas9 proteins have been widely applied in
programmable genome recombination, base editing or gene regulation in both prokaryotes …
programmable genome recombination, base editing or gene regulation in both prokaryotes …
On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
The ability to block gene expression in bacteria with the catalytically inactive mutant of Cas9,
known as dCas9, is quickly becoming a standard methodology to probe gene function …
known as dCas9, is quickly becoming a standard methodology to probe gene function …
The genetics of aerotolerant growth in an alphaproteobacterium with a naturally reduced genome
Reduced genome bacteria are genetically simplified systems that facilitate biological study
and industrial use. The free-living alphaproteobacterium Zymomonas mobilis has a naturally …
and industrial use. The free-living alphaproteobacterium Zymomonas mobilis has a naturally …
The Rcs stress response inversely controls surface and CRISPR–Cas adaptive immunity to discriminate plasmids and phages
Bacteria harbour multiple innate defences and adaptive CRISPR–Cas systems that provide
immunity against bacteriophages and mobile genetic elements. Although some bacteria …
immunity against bacteriophages and mobile genetic elements. Although some bacteria …
A high-efficacy CRISPR interference system for gene function discovery in Zymomonas mobilis
Zymomonas mobilis is a promising biofuel producer due to its high alcohol tolerance and
streamlined metabolism that efficiently converts sugar to ethanol. Z. mobilis genes are poorly …
streamlined metabolism that efficiently converts sugar to ethanol. Z. mobilis genes are poorly …
Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement
The RNA-guided nuclease Cas9 has unlocked powerful methods for perturbing both the
genome through targeted DNA cleavage and the regulome through targeted DNA binding …
genome through targeted DNA cleavage and the regulome through targeted DNA binding …
[HTML][HTML] Mapping the functional landscape of the receptor binding domain of T7 bacteriophage by deep mutational scanning
The interaction between a bacteriophage and its host is mediated by the phage's receptor
binding protein (RBP). Despite its fundamental role in governing phage activity and host …
binding protein (RBP). Despite its fundamental role in governing phage activity and host …
Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coli
Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular
systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each …
systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each …
[HTML][HTML] Comprehensive genome-wide perturbations via CRISPR adaptation reveal complex genetics of antibiotic sensitivity
W Jiang, P Oikonomou, S Tavazoie - Cell, 2020 - cell.com
Genome-wide CRISPR screens enable systematic interrogation of gene function. However,
guide RNA libraries are costly to synthesize, and their limited diversity compromises the …
guide RNA libraries are costly to synthesize, and their limited diversity compromises the …