Systematic genome-wide querying of coding and non-coding functional elements in E. coli using CRISPRi
Genome-wide repression screens using CRISPR interference (CRISPRi) have enabled the
high-throughput identification of essential genes in bacteria. However, there is a lack of …
high-throughput identification of essential genes in bacteria. However, there is a lack of …
Comparative genomics hints at dispensability of multiple essential genes in two Escherichia coli L-form strains
Y Liu, Y Zhang, C Kang, D Tian, H Lu, B Xu… - Bioscience …, 2023 - portlandpress.com
Despite the critical role of bacterial cell walls in maintaining cell shapes, certain
environmental stressors can induce the transition of many bacterial species into a wall …
environmental stressors can induce the transition of many bacterial species into a wall …
Revealing causes for false-positive and false-negative calling of gene essentiality in Escherichia coli using transposon insertion sequencing
The massive sequencing of transposon insertion mutant libraries (Tn-Seq) represents a
commonly used method to determine essential genes in bacteria. Using a hypersaturated …
commonly used method to determine essential genes in bacteria. Using a hypersaturated …
A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli
Advances in gene editing, in particular CRISPR interference (CRISPRi), have enabled
depletion of essential cellular machinery to study the downstream effects on bacterial …
depletion of essential cellular machinery to study the downstream effects on bacterial …
[HTML][HTML] Identification of essential genes that support fitness of Acinetobacter baumannii efflux pump overproducers in the presence of fluoroquinolone
E Hamami, W Huo, K Neal, I Neisewander, E Geisinger… - bioRxiv, 2024 - ncbi.nlm.nih.gov
Acinetobacter baumannii is a nosocomial pathogen often associated with multidrug
resistance (MDR) infections. Fluoroquinolone resistance (FQR) due to drug target site …
resistance (MDR) infections. Fluoroquinolone resistance (FQR) due to drug target site …
[HTML][HTML] Recent advances in genome-scale engineering in Escherichia coli and their applications
H Gao, Z Qiu, X Wang, X Zhang, Y Zhang, J Dai… - Engineering …, 2023 - Elsevier
Owing to the rapid advancement of genome engineering technologies, the scale of genome
engineering has expanded dramatically. Genome editing has progressed from one genomic …
engineering has expanded dramatically. Genome editing has progressed from one genomic …
Past, Present, and Future of Genome Modification in Escherichia coli
H Mori, M Kataoka, X Yang - Microorganisms, 2022 - mdpi.com
Escherichia coli K-12 is one of the most well-studied species of bacteria. This species,
however, is much more difficult to modify by homologous recombination (HR) than other …
however, is much more difficult to modify by homologous recombination (HR) than other …
CRISPR Interference-Based Functional Small RNA Genomics
G Prezza, AJ Westermann - Bacterial Regulatory RNA: Methods and …, 2024 - Springer
Small RNAs (sRNAs) are versatile regulators universally present in species across the
prokaryotic kingdom, yet their functional characterization remains a major bottleneck. Gene …
prokaryotic kingdom, yet their functional characterization remains a major bottleneck. Gene …
A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli
CRISPR technologies have become standard laboratory tools for genetic manipulations
across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR …
across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR …
Three-dimensional biofilm colony growth supports a mutualism involving matrix and nutrient sharing
Life in a three-dimensional biofilm is typical for many bacteria, yet little is known about how
strains interact in this context. Here, we created essential gene CRISPR interference …
strains interact in this context. Here, we created essential gene CRISPR interference …