Spontaneous mouse models of cancer show promise to more accurately recapitulate human
disease and predict clinical efficacy. Transgenic mice or viral vectors have been required to …

Sleeping Beauty (SB 3) transposon and transposase constitute a DNA plasmid system used
for therapeutic human cell genetic engineering. Here we report a comparison of SB100X, a …

Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for
expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system …

Abstract The Sleeping Beauty and PiggyBac DNA transposon systems have recently been
developed as tools for insertional mutagenesis. We have compared the chromosomal …

Effective gene therapy requires robust delivery of the desired genes into the relevant target
cells, long-term gene expression, and minimal risks of secondary effects. The development …

DNA transposons are primitive genetic elements which have colonized living organisms
from plants to bacteria and mammals. Through evolution such parasitic elements have …

Recent results confirm that long‐term expression of therapeutic transgenes can be achieved
by using a transposon‐based system in primary stem cells and in vivo. Transposable …

Current techniques for the genetic engineering of insect genomes utilize transposable
genetic elements, which are inefficient, have limited carrying capacity and give rise to …

Transposon-based vectors represent promising new tools for chromosomal transgene
insertion and establishment of persistent gene expression in vivo. Here, we report the …

Hemophilia A is a lead candidate for treatment by gene therapy because small increments in
the missing secreted protein product, coagulation factor VIII (FVIII), would result in …