Quantification of E. coli DNA on a Flow-through Chemiluminescence Microarray Readout System after PCR Amplification

SC Donhauser, R Niessner, M Seidel - Analytical Sciences, 2009 - Springer
We report on a hybridization assay using DNA microarrays for the quantification of
amplification products of the uidA gene of E. coli. Using the stopped-PCR strategy, the …

LA35 poultry fecal marker persistence is correlated with that of indicators and pathogens in environmental waters

B Nayak, J Weidhaas, VJ Harwood - Applied and Environmental …, 2015 - Am Soc Microbiol
Disposal of fecally contaminated poultry litter by land application can deliver pathogens and
fecal indicator bacteria (FIB) into receiving waters via runoff. While water quality is regulated …

[PDF][PDF] Development of an absolute quantitative real-time PCR (qPCR) for the diagnosis of Aeromonas hydrophila infections in fish

F Sebastiao, EM Lemos, F Pilarski - Acta Sci. Microbiol, 2018 - researchgate.net
Absolute quantitative real-time PCR (qPCR), a variation of polymerase chain reaction
(PCR), has been used to identify Aeromonas spp. in wastewater effluent and catfish [8, 9] …

[HTML][HTML] Assessing the accuracy of quantitative molecular microbial profiling

DM O'Sullivan, T Laver, S Temisak, N Redshaw… - International Journal of …, 2014 - mdpi.com
The application of high-throughput sequencing in profiling microbial communities is
providing an unprecedented ability to investigate microbiomes. Such studies typically apply …

Simultaneous detection of selected enteric viruses in water samples by multiplex quantitative PCR

DY Lee, KT Leung, H Lee, MB Habash - Water, air, & soil pollution, 2016 - Springer
Abstract Human rotavirus (HRV), adenovirus (HAdV), and norovirus (HNV) are the most
common causes of viral gastroenteritis in humans worldwide. Application of quantitative …

A comparison of nonlethal and destructive methods for broad‐based infectious agent screening of Chinook Salmon using high‐throughput qPCR

AK Teffer, KM Miller - Journal of Aquatic Animal Health, 2019 - Wiley Online Library
Molecular tools, such as high‐throughput quantitative polymerase chain reaction (HT‐
qPCR), are useful for monitoring multiple infectious agents in wild animal populations (ie …

Operation performance and microbial community dynamics of phosphorus removal sludge with different electron acceptors

X Lv, M Shao, C Li, J Li, D Liu, X Gao… - Journal of Industrial …, 2014 - academic.oup.com
Operation performances of phosphorus removal sludge with different electron acceptors in
three parallel SBRs were firstly compared in the present study, and the effect of post …

A Method Based on a Modified Fluorescence In Situ Hybridization (FISH) Approach for the Sensing of Staphylococcus aureus from Nasal Samples

AJ Sami, S Bilal, S Alam, M Khalid… - Applied Biochemistry and …, 2024 - Springer
Staphylococcus aureus is a major source of bacteremia and develops several
complications, causing high morbidity and mortality. Rapid identification and detection of …

Advantages and disadvantages of microarrays to study microbial population dynamics a minireview

KR Everett, J Rees-George, IPS Pushparajah… - New Zealand Plant …, 2010 - nzpps.org
Microbial ecology is challenging because of practical problems associated with detecting
and quantifying populations Bacteria and yeasts are not easily identified using conventional …

Enumeration of methanogens with a focus on fluorescence in situ hybridization

S Kumar, SS Dagar, AK Mohanty, SK Sirohi… - …, 2011 - Springer
Methanogens, the members of domain Archaea are potent contributors in global warming.
Being confined to the strict anaerobic environment, their direct cultivation as pure culture is …