Theory and simulation of multiphase coexistence in biomolecular mixtures

WM Jacobs - Journal of Chemical Theory and Computation, 2023 - ACS Publications
Biomolecular condensates constitute a newly recognized form of spatial organization in
living cells. Although many condensates are believed to form as a result of phase …

Fundamental Aspects of Phase-Separated Biomolecular Condensates

HX Zhou, D Kota, S Qin, R Prasad - Chemical Reviews, 2024 - ACS Publications
Biomolecular condensates, formed through phase separation, are upending our
understanding in much of molecular, cell, and developmental biology. There is an urgent …

[HTML][HTML] Phase separation of protein mixtures is driven by the interplay of homotypic and heterotypic interactions

M Farag, WM Borcherds, A Bremer, T Mittag… - Nature …, 2023 - nature.com
Prion-like low-complexity domains (PLCDs) are involved in the formation and regulation of
distinct biomolecular condensates that form via phase separation coupled to percolation …

Determinants that enable disordered protein assembly into discrete condensed phases

RM Welles, KA Sojitra, MV Garabedian, B Xia… - Nature Chemistry, 2024 - nature.com
Cells harbour numerous mesoscale membraneless compartments that house specific
biochemical processes and perform distinct cellular functions. These protein-and RNA-rich …

[HTML][HTML] Quantitative real-time in-cell imaging reveals heterogeneous clusters of proteins prior to condensation

C Lan, J Kim, S Ulferts, F Aprile-Garcia… - Nature …, 2023 - nature.com
Our current understanding of biomolecular condensate formation is largely based on
observing the final near-equilibrium condensate state. Despite expectations from classical …

[HTML][HTML] Interplay between charge distribution and DNA in shaping HP1 paralog phase separation and localization

TM Phan, YC Kim, GT Debelouchina, J Mittal - Elife, 2024 - elifesciences.org
Abstract The heterochromatin protein 1 (HP1) family is a crucial component of
heterochromatin with diverse functions in gene regulation, cell cycle control, and cell …

[HTML][HTML] Aromatic and arginine content drives multiphasic condensation of protein-RNA mixtures

PY Chew, JA Joseph, R Collepardo-Guevara… - Biophysical …, 2024 - cell.com
Multiphasic architectures are found ubiquitously in biomolecular condensates and are
thought to have important implications for the organization of multiple chemical reactions …

Determinants of disordered protein co-assembly into discrete condensed phases

RM Welles, KA Sojitra, MV Garabedian, B Xia… - bioRxiv, 2023 - biorxiv.org
Cells harbor numerous mesoscale membraneless compartments that house specific
biochemical processes and perform distinct cellular functions. These protein and RNA-rich …

Programmable phase behavior in fluids with designable interactions

F Chen, WM Jacobs - The Journal of Chemical Physics, 2023 - pubs.aip.org
We introduce a method for solving the “inverse” phase equilibria problem: How should the
interactions among a collection of molecular species be designed in order to achieve a …

[HTML][HTML] Phase separation in mixtures of prion-like low complexity domains is driven by the interplay of homotypic and heterotypic interactions

R Pappu, M Farag, W Borcherds, A Bremer… - Research …, 2023 - ncbi.nlm.nih.gov
Prion-like low-complexity domains (PLCDs) are involved in the formation and regulation of
distinct biomolecular condensates that form via coupled associative and segregative phase …