[HTML][HTML] Methodology for fish biodiversity monitoring with environmental DNA metabarcoding: The primers, databases and bioinformatic pipelines
Environmental DNA (eDNA) originates from cellular material shed by organisms into aquatic
or terrestrial environments and can be sampled and monitored using metabarcoding …
or terrestrial environments and can be sampled and monitored using metabarcoding …
Navigating the seven challenges of taxonomic reference databases in metabarcoding analyses
Assessment of biodiversity using metabarcoding data, such as from bulk or environmental
DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and …
DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and …
Comparing environmental DNA metabarcoding and underwater visual census to monitor tropical reef fishes
Environmental DNA (eDNA) analysis is a revolutionary method to monitor marine
biodiversity from animal DNA traces. Examining the capacity of eDNA to provide accurate …
biodiversity from animal DNA traces. Examining the capacity of eDNA to provide accurate …
Benchmarking bioinformatic tools for fast and accurate eDNA metabarcoding species identification
L Mathon, A Valentini, PE Guérin… - Molecular Ecology …, 2021 - Wiley Online Library
Bioinformatic analysis of eDNA metabarcoding data is a crucial step toward rigorously
assessing biodiversity. Many programs are now available for each step of the required …
assessing biodiversity. Many programs are now available for each step of the required …
Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary
Metabarcoding of environmental DNA is increasingly used for biodiversity assessments in
aquatic communities. The efficiency and outcome of these efforts are dependent upon either …
aquatic communities. The efficiency and outcome of these efforts are dependent upon either …
Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems
A Polanco F, E Richards, B Flück, A Valentini… - Environmental …, 2021 - Wiley Online Library
Through the development of environmental DNA (eDNA) metabarcoding, in situ monitoring
of organisms is becoming easier and promises a revolution in our approaches to detect …
of organisms is becoming easier and promises a revolution in our approaches to detect …
Cross-ocean patterns and processes in fish biodiversity on coral reefs through the lens of eDNA metabarcoding
Increasing speed and magnitude of global change threaten the world's biodiversity and
particularly coral reef fishes. A better understanding of large-scale patterns and processes …
particularly coral reef fishes. A better understanding of large-scale patterns and processes …
Use of environmental DNA in assessment of fish functional and phylogenetic diversity
V Marques, P Castagné, A Polanco… - Conservation …, 2021 - Wiley Online Library
Assessing the impact of global changes and protection effectiveness is a key step in
monitoring marine fishes. Most traditional census methods are demanding or destructive …
monitoring marine fishes. Most traditional census methods are demanding or destructive …
Be positive: customized reference databases and new, local barcodes balance false taxonomic assignments in metabarcoding studies
F Mugnai, F Costantini, A Chenuil, M Leduc… - PeerJ, 2023 - peerj.com
Background In metabarcoding analyses, the taxonomic assignment is crucial to place
sequencing data in biological and ecological contexts. This fundamental step depends on a …
sequencing data in biological and ecological contexts. This fundamental step depends on a …
How many replicates to accurately estimate fish biodiversity using environmental DNA on coral reefs?
Quantifying fish species diversity in rich tropical marine environments remains challenging.
Environmental DNA (eDNA) metabarcoding is a promising tool to face this challenge …
Environmental DNA (eDNA) metabarcoding is a promising tool to face this challenge …