[HTML][HTML] Recent developments in bioprocessing of recombinant proteins: expression hosts and process development
NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …
humans worldwide. The production of therapeutic proteins for treating diseases at large …
Fusion tags to enhance heterologous protein expression
MR Ki, SP Pack - Applied microbiology and biotechnology, 2020 - Springer
Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …
Microbial protein cell factories fight back?
LA Rettenbacher, K Arauzo-Aguilera, L Buscajoni… - Trends in …, 2022 - cell.com
The biopharmaceutical market is growing faster than ever, with two production systems
competing for market dominance: mammalian cells and microorganisms. In recent years …
competing for market dominance: mammalian cells and microorganisms. In recent years …
Drug repurposing for the SARS‐CoV‐2 papain‐like protease
As the pathogen of COVID‐19, SARS‐CoV‐2 encodes two essential cysteine proteases that
process the pathogen's two large polypeptide products pp1a and pp1ab in the human cell …
process the pathogen's two large polypeptide products pp1a and pp1ab in the human cell …
Immunoaffinity biosensors for the detection of SARS-CoV-1 using screened Fv-antibodies from an autodisplayed Fv-antibody library
J Jung, JH Bong, JS Sung, JH Park, TH Kim… - Biosensors and …, 2023 - Elsevier
The detection of severe acute respiratory syndrome coronavirus (SARS-CoV-1) was
demonstrated using screened Fv-antibodies for SPR biosensor and impedance …
demonstrated using screened Fv-antibodies for SPR biosensor and impedance …
Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor
KK Haridhasapavalan, SH Ranjan… - Applied Microbiology …, 2021 - Springer
Transcription factor MESP1 is a crucial factor regulating cardiac, hematopoietic, and skeletal
myogenic development. Besides, it also contributes to the generation of functional …
myogenic development. Besides, it also contributes to the generation of functional …
Effects of variable domain orientation on anti‐HER2 single‐chain variable fragment antibody expressed in the Escherichia coli cytoplasm
Single‐chain variable fragment (scFv) antibodies have great potential for a range of
applications including as diagnostic and therapeutic agents. However, production of scFvs is …
applications including as diagnostic and therapeutic agents. However, production of scFvs is …
A one-step immunoassay based on switching peptides for diagnosis of porcine epidemic diarrhea virus (PEDV) using screened Fv-antibodies
TH Kim, JY Park, J Jung, JS Sung, S Kwon… - Journal of Materials …, 2024 - pubs.rsc.org
In this study, a one-step immunoassay for porcine epidemic diarrhea virus (PEDV) based on
Fv-antibodies and switching peptides was developed, and the assay results of PEDV were …
Fv-antibodies and switching peptides was developed, and the assay results of PEDV were …
[HTML][HTML] 1Progress, applications, challenges and prospects of protein purification technology
M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …
role in cell growth, development, movement, heredity, reproduction and other life activities …
Colorimetric and chemiluminescent enzyme immunoassays based on the alkaline phosphatase-tagged single-chain variable fragment fusion tracer for detecting …
X Liu, J Li, R Chen, X Xie, F Mao, Z Sun, Z He, H Cao… - Food Chemistry, 2024 - Elsevier
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin and seriously threatens food
safety, which requires rapid and sensitive detection methods for monitoring ZEN in agro …
safety, which requires rapid and sensitive detection methods for monitoring ZEN in agro …