[HTML][HTML] Recent developments in bioprocessing of recombinant proteins: expression hosts and process development

NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Fusion tags to enhance heterologous protein expression

MR Ki, SP Pack - Applied microbiology and biotechnology, 2020 - Springer
Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …

Microbial protein cell factories fight back?

LA Rettenbacher, K Arauzo-Aguilera, L Buscajoni… - Trends in …, 2022 - cell.com
The biopharmaceutical market is growing faster than ever, with two production systems
competing for market dominance: mammalian cells and microorganisms. In recent years …

Drug repurposing for the SARS‐CoV‐2 papain‐like protease

CC Cho, SG Li, TJ Lalonde, KS Yang, G Yu… - …, 2022 - Wiley Online Library
As the pathogen of COVID‐19, SARS‐CoV‐2 encodes two essential cysteine proteases that
process the pathogen's two large polypeptide products pp1a and pp1ab in the human cell …

Immunoaffinity biosensors for the detection of SARS-CoV-1 using screened Fv-antibodies from an autodisplayed Fv-antibody library

J Jung, JH Bong, JS Sung, JH Park, TH Kim… - Biosensors and …, 2023 - Elsevier
The detection of severe acute respiratory syndrome coronavirus (SARS-CoV-1) was
demonstrated using screened Fv-antibodies for SPR biosensor and impedance …

Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor

KK Haridhasapavalan, SH Ranjan… - Applied Microbiology …, 2021 - Springer
Transcription factor MESP1 is a crucial factor regulating cardiac, hematopoietic, and skeletal
myogenic development. Besides, it also contributes to the generation of functional …

Effects of variable domain orientation on anti‐HER2 single‐chain variable fragment antibody expressed in the Escherichia coli cytoplasm

İ Koçer, EC Cox, MP DeLisa, E Çelik - Biotechnology progress, 2021 - Wiley Online Library
Single‐chain variable fragment (scFv) antibodies have great potential for a range of
applications including as diagnostic and therapeutic agents. However, production of scFvs is …

A one-step immunoassay based on switching peptides for diagnosis of porcine epidemic diarrhea virus (PEDV) using screened Fv-antibodies

TH Kim, JY Park, J Jung, JS Sung, S Kwon… - Journal of Materials …, 2024 - pubs.rsc.org
In this study, a one-step immunoassay for porcine epidemic diarrhea virus (PEDV) based on
Fv-antibodies and switching peptides was developed, and the assay results of PEDV were …

[HTML][HTML] 1Progress, applications, challenges and prospects of protein purification technology

M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …

Colorimetric and chemiluminescent enzyme immunoassays based on the alkaline phosphatase-tagged single-chain variable fragment fusion tracer for detecting …

X Liu, J Li, R Chen, X Xie, F Mao, Z Sun, Z He, H Cao… - Food Chemistry, 2024 - Elsevier
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin and seriously threatens food
safety, which requires rapid and sensitive detection methods for monitoring ZEN in agro …