Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays

SN Rampersad - Sensors, 2012 - mdpi.com
Accurate prediction of the adverse effects of test compounds on living systems, detection of
toxic thresholds, and expansion of experimental data sets to include multiple toxicity end …

Testing of antifungal natural products: methodologies, comparability of results and assay choice

F Hadacek, H Greger - Phytochemical Analysis: An …, 2000 - Wiley Online Library
Antifungal testing of filamentous fungi generally suffers from incomparability of results. In
order to illustrate this problem, the polyacetylene falcarindiol and the naphthoquinone …

In vitro cytotoxicity and cell viability assays: principles, advantages, and disadvantages

ÖS Aslantürk - Genotoxicity-A predictable risk to our actual world, 2018 - books.google.com
Cytotoxicity is one of the most important indicators for biological evaluation in vitro studies. In
vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such …

A method of high-throughput functional evaluation of EGFR gene variants of unknown significance in cancer

S Kohsaka, M Nagano, T Ueno, Y Suehara… - Science translational …, 2017 - science.org
Numerous variants of unknown significance (VUS) have been identified through large-scale
cancer genome projects, although their functional relevance remains uninvestigated. We …

Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity

J O'brien, I Wilson, T Orton… - European journal of …, 2000 - Wiley Online Library
We show here the identity of Alamar Blue as resazurin. The 'resazurin reduction test'has
been used for about 50 years to monitor bacterial and yeast contamination of milk, and also …

A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H] thymidine incorporation assay

SA Ahmed, RM Gogal Jr, JE Walsh - Journal of immunological methods, 1994 - Elsevier
A one-step non-radioactive assay to determine the proliferation of murine lymphocytes,
lymphoid tumor cells and hybridoma cells is described. This assay requires the addition of …

Comparison of alamar blue and MTT assays for high through-put screening

R Hamid, Y Rotshteyn, L Rabadi, R Parikh, P Bullock - Toxicology in vitro, 2004 - Elsevier
The performance of alamar blue and 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium
bromide (MTT) cell viability assays in a high through-put format were compared. A total of …

The Alamar Blue® assay to determine drug sensitivity of African trypanosomes (Tb rhodesiense and Tb gambiense) in vitro

B Räz, M Iten, Y Grether-Bühler, R Kaminsky, R Brun - Acta tropica, 1997 - Elsevier
Alamar Blue®, an indicator for metabolic cell function, was evaluated as a fluorescent and
as a colorimetric dye in drug sensitivity assays for human pathogenic African trypanosomes …

Effect of fermentation time on the content of bioactive compounds with cosmetic and dermatological properties in Kombucha Yerba Mate extracts

A Ziemlewska, Z Nizioł-Łukaszewska, T Bujak… - Scientific Reports, 2021 - nature.com
Kombucha is a beverage made by fermenting sugared tea using a symbiotic culture of
bacteria belonging to the genus Acetobacter, Gluconobacter, and the yeasts of the genus …

Replacement, reduction, and refinement of animal experiments in anticancer drug development: the contribution of 3D in vitro cancer models in the drug efficacy …

EM Tosca, D Ronchi, D Facciolo, P Magni - Biomedicines, 2023 - mdpi.com
In the last decades three-dimensional (3D) in vitro cancer models have been proposed as a
bridge between bidimensional (2D) cell cultures and in vivo animal models, the gold …