[HTML][HTML] Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes

MTJ Johnson, EJ Carpenter, Z Tian, R Bruskiewich… - PloS one, 2012 - journals.plos.org
Next-generation sequencing plays a central role in the characterization and quantification of
transcriptomes. Although numerous metrics are purported to quantify the quality of RNA …

[HTML][HTML] Isolation of high-quality RNA from Platycladus orientalis and other Cupressaceae plants

E Chang, Y Zhao, Q Wei, S Shi, Z Jiang - Electronic Journal of …, 2016 - Elsevier
Platycladus orientalis has a lifespan of several thousand years in China, making it a good
plant in which to study aging at the molecular level, but this requires sufficient quantities of …

[HTML][HTML] How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping …

E De Keyser, L Desmet, E Van Bockstaele… - BMC molecular …, 2013 - Springer
Background Flower colour variation is one of the most crucial selection criteria in the
breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii …

Evaluation of different RNA extraction methods of filamentous fungi in various food matrices

V Bernáldez, A Rodríguez, M Rodriguez… - LWT, 2017 - Elsevier
Aflatoxin producing-moulds may grow on different foods (eg cereals, ripened meats) and
contaminate such products with aflatoxins. Molecular studies about expression of genes …

A protocol for extraction of high-quality RNA and DNA from peanut plant tissues

D Yin, H Liu, X Zhang, D Cui - Molecular biotechnology, 2011 - Springer
Peanuts are an increasingly important global food source. However, until recently the lack of
effective protocols for the extraction of nucleic acids has made molecular studies of peanut …

Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica

YP de Coaña, N Parody, MÁ Fuertes, J Carnés… - Biochemical and …, 2010 - Elsevier
Sensitization to Cupressaceae pollen has become one of the most important causes of
pollinosis in Western countries during winter and early spring. However, the characterization …

[PDF][PDF] An effective protocol for isolation of high-quality RNA from pomegranate seeds

A Zarei, Z Zamani, A Mousavi, R Fatahi… - Asian Aust J Plant Sci …, 2012 - academia.edu
In the present study, various protocols were tested to extract RNA from different parts of
pomegranate, especially the fruit. Using a modified CTAB-based procedure, high quality …

High RNA quality extracted from the tolerant crop Cyamopsis tetragonoloba (L.) despite possession of low RNA integrity number

ARZ Gaafar, F Al-Qurainy, A Alshameri… - Biotechnology & …, 2021 - Taylor & Francis
Salinity is one of the most limiting constraints of crop production. Nevertheless, highly-
tolerant crop plants (eg 'Guar'Cyamopsis tetragonoloba) can thrive well under salinity …

High-Quality RNA Preparation from Rhodosporidium toruloides and cDNA Library Construction Therewith

F Yang, H Tan, Y Zhou, X Lin, S Zhang - Molecular biotechnology, 2011 - Springer
Oleaginous yeast Rhodosporidium toruloides is an excellent microbial lipid producer.
Therefore, it is important to develop molecular biology tools to understand the basic …

Isolation of high-quality rna from grains of different maize varieties

RS Messias, V Galli, JH Buss, JM Borowski… - Preparative …, 2014 - Taylor & Francis
The study of gene expression in maize varieties represents a powerful tool aiming to
increase vitamin A precursors. However, the isolation of RNA from different maize varieties …