Pseudomonas aeruginosa is an opportunistic pathogen affecting immunocompromised
patients. It is known as the leading cause of morbidity and mortality in cystic fibrosis (CF) …

The ability to construct and maintain a structured multicellular bacterial community depends
critically on the production of extracellular matrix components (1, 2). While the biofilm matrix …

The cyclic di-GMP (c-di-GMP) second messenger represents a signaling system that
regulates many bacterial behaviors and is of key importance for driving the lifestyle switch …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single …

Bacterial biofilm formation is dependent mainly on the decision-making process of the two
key factors of the gene regulatory network, namely the Quorum Sensing (QS) system and bis …

Twenty-five years have passed since the discovery of cyclic dimeric (3′→ 5′) GMP (cyclic
di-GMP or c-di-GMP). From the relative obscurity of an allosteric activator of a bacterial …

Many bacteria swim in liquid or swarm over solid surfaces by synthesizing rotary flagella.
The same bacteria that are motile also commonly form nonmotile multicellular aggregates …

Since its initial discovery as an allosteric factor regulating cellulose biosynthesis in
Gluconacetobacter xylinus, the list of functional outputs regulated by c-di-GMP has grown …

Pseudomonas aeruginosa is a metabolically versatile bacterium that is found in a wide
range of biotic and abiotic habitats. It is a major human opportunistic pathogen causing …

The striking multiplicity, signal input diversity, and output specificity of c-di-GMP signaling
proteins in many bacteria has brought second messenger signaling back onto the agenda of …