Effects of different cryopreservation methods on DNA integrity and sperm chromatin quality in men

MF Lusignan, X Li, B Herrero, G Delbès, PTK Chan - Andrology, 2018 - Wiley Online Library
Background Cryopreserved human sperm are used in assisted reproductive technology.
However, the effect of cryopreservation on sperm DNA integrity is unclear. Objectives The …

Semen cryopreservation in dogs and foxes

W Farstad - Animal Reproduction Science, 1996 - Elsevier
Low temperature preservation of canid semen has been a subject of increasing interest
among dog breeders, fur breeders and scientists. Research has focused on the use of …

Canine fresh and cryopreserved semen evaluation

AIP Martı́nez - Animal reproduction science, 2004 - Elsevier
There is little doubt that objective assessment of multiple parameters related to the functional
and morphological characteristics of spermatozoa, increase the predictability of the fertilizing …

Freeze–thaw induced genotoxicity in buffalo (Bubalus bubalis) spermatozoa in relation to total antioxidant status

R Kumar, G Jagan Mohanarao, Arvind… - Molecular biology …, 2011 - Springer
Use of cryopreserved semen has become an important tool in assisted reproduction but
freezing and thawing cause sub-lethal damage to spermatozoa. This is detrimental to sperm …

Effects of spermatozoal concentration and post-thaw dilution rate on survival after thawing of dog spermatozoa

A Peña, C Linde-Forsberg - Theriogenology, 2000 - Elsevier
The objectives of this study were to evaluate the effects and interactions of freezing dog
semen using 4 different sperm concentrations (50× 106, 100× 106, 200× 106 and 400× 106 …

Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender

A Najafi, M Zhandi, A Towhidi, M Sharafi, AA Sharif… - Cryobiology, 2013 - Elsevier
The objective of this study was to examine the interaction of different concentrations of
trehalose [0 (T0), 50 (T50) or 100 (T100) mM] and glycerol [5%(G5) or 7%(G7)] on post …

Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation

P Kumar, D Kumar, P Sikka, P Singh - Animal reproduction science, 2015 - Elsevier
The variety of mammalian cells has been successfully cryopreserved by use of the silk
protein sericin due to its strong free-radical-scavenging and potent antioxidant activity. The …

Cryopreservation of turbot (Scophthalmus maximus) spermatozoa

C Dreanno, M Suquet, L Quemener, J Cosson… - Theriogenology, 1997 - Elsevier
The aim of this study was to develop a method for cryopreserving turbot semen and to
compare sperm motility characteristics, metabolic status and fertilization capacity of …

Addition of autologous platelet rich plasma to semen extender enhances cryotolerance and fertilizing capacity of buffalo bull spermatozoa

HR El-Sherbiny, EA Abdelnaby, H Samir, M Fathi - Theriogenology, 2022 - Elsevier
Platelet rich plasma (PRP) is extensively used in regenerative medicine. Present work was
aimed to investigate the effect of autologous PRP supplementation in semen freezing …

Assessment of sperm cryodamage and strategies to improve outcome

S Oehninger, NK Duru, C Srisombut… - Molecular and Cellular …, 2000 - Elsevier
Sperm cryopreservation still represents a valuable clinical aid in the management of
infertility. Its current principal indications include (1) donor sperm insemination;(2) freezing …