Review of CRISPR/Cas9 sgRNA design tools
The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Genomes in focus: development and applications of CRISPR‐Cas9 imaging technologies
SC Knight, R Tjian, JA Doudna - … Chemie International Edition, 2018 - Wiley Online Library
The discovery of the CRISPR‐Cas9 endonuclease has enabled facile genome editing in
living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind …
living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind …
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo
The combination of Cas9, guide RNA and repair template DNA can induce precise gene
editing and the correction of genetic diseases in adult mammals. However, clinical …
editing and the correction of genetic diseases in adult mammals. However, clinical …
Off-target predictions in CRISPR-Cas9 gene editing using deep learning
Motivation The prediction of off-target mutations in CRISPR-Cas9 is a hot topic due to its
relevance to gene editing research. Existing prediction methods have been developed; …
relevance to gene editing research. Existing prediction methods have been developed; …
DNA-binding-domain fusions enhance the targeting range and precision of Cas9
MF Bolukbasi, A Gupta, S Oikemus, AG Derr… - Nature …, 2015 - nature.com
The CRISPR-Cas9 system is commonly used in biomedical research; however, the
precision of Cas9 is suboptimal for applications that involve editing a large population of …
precision of Cas9 is suboptimal for applications that involve editing a large population of …
[HTML][HTML] Impact of CRISPR-Cas9-based genome engineering in farm animals
Humans are sorely over-dependent on livestock for their daily basic need of food in the form
of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the …
of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the …
A review of CRISPR associated genome engineering: application, advances and future prospects of genome targeting tool for crop improvement
The Cas9 nuclease initiates double-stranded breaks at the target position in DNA, which are
repaired by the intracellular restoration pathways to eliminate or insert pieces of DNA …
repaired by the intracellular restoration pathways to eliminate or insert pieces of DNA …
[HTML][HTML] Viral diversity is an obligate consideration in CRISPR/Cas9 designs for targeting the HIV reservoir
P Roychoudhury, H De Silva Feelixge, D Reeves… - BMC biology, 2018 - Springer
Abstract Background RNA-guided CRISPR/Cas9 systems can be designed to mutate or
excise the integrated HIV genome from latently infected cells and have therefore been …
excise the integrated HIV genome from latently infected cells and have therefore been …
[HTML][HTML] The prominent characteristics of the effective sgRNA for a precise CRISPR genome editing
R Mohammadhassan, S Tutunchi… - CRISPR Technology …, 2022 - intechopen.com
Clustered regularly interspaced short palindromic repeats (CRISPRs) technique is the most
effective and novelist technique for genome editing. CRISPR mechanism has been widely …
effective and novelist technique for genome editing. CRISPR mechanism has been widely …
Cas9-DNA targeting unit chimeras
SA Wolfe, MF Bolukbasi, A Gupta… - US Patent …, 2019 - Google Patents
2016-02-18 Assigned to UNIVERSITY OF MASSACHUSETTS reassignment UNIVERSITY
OF MASSACHUSETTS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …
OF MASSACHUSETTS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR …