Targeting p53 pathways: mechanisms, structures, and advances in therapy
H Wang, M Guo, H Wei, Y Chen - Signal transduction and targeted …, 2023 - nature.com
The TP53 tumor suppressor is the most frequently altered gene in human cancers, and has
been a major focus of oncology research. The p53 protein is a transcription factor that can …
been a major focus of oncology research. The p53 protein is a transcription factor that can …
Protein folds vs. protein folding: Differing questions, different challenges
Protein fold prediction using deep-learning artificial intelligence (AI) has transformed the
field of protein structure prediction (1–3). By combining physical and geometric constraints …
field of protein structure prediction (1–3). By combining physical and geometric constraints …
Functional and biochemical characterization of the MazEF6 toxin-antitoxin system of Mycobacterium tuberculosis
The Mycobacterium tuberculosis genome harbors nine toxin-antitoxin (TA) systems that are
members of the mazEF family, unlike other prokaryotes, which have only one or two …
members of the mazEF family, unlike other prokaryotes, which have only one or two …
Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries
Although there have been recent transformative advances in the area of protein structure
prediction, prediction of point mutations that improve protein stability remains challenging. It …
prediction, prediction of point mutations that improve protein stability remains challenging. It …
A CcdB toxin‐derived peptide acts as a broad‐spectrum antibacterial therapeutic in infected mice
Abstract The bacterial toxin CcdB (Controller of Cell death or division B) targets DNA
Gyrase, an essential bacterial topoisomerase, which is also the molecular target for …
Gyrase, an essential bacterial topoisomerase, which is also the molecular target for …
Deep mutational scanning to probe specificity determinants in proteins
J Bhowmick, S Chandra, R Varadarajan - Structure and Intrinsic Disorder in …, 2023 - Elsevier
Deep mutational scanning (DMS) allows massively parallel investigations of large libraries
of gene variants and helps in elucidating genotype–function–stability relationships in …
of gene variants and helps in elucidating genotype–function–stability relationships in …
Structural and functional determinants inferred from deep mutational scans
Mutations that affect protein binding to a cognate partner primarily occur either at buried
residues or at exposed residues directly involved in partner binding. Distinguishing between …
residues or at exposed residues directly involved in partner binding. Distinguishing between …
Combining cysteine scanning with chemical labeling to map protein-protein interactions and infer bound structure in an intrinsically disordered region
The Mycobacterium tuberculosis genome harbours nine toxin-antitoxin (TA) systems of the
mazEF family. These consist of two proteins, a toxin and an antitoxin, encoded in an operon …
mazEF family. These consist of two proteins, a toxin and an antitoxin, encoded in an operon …
Biophysical Correlates of Enhanced Immunogenicity of a Stabilized Variant of the Receptor Binding Domain of SARS-CoV-2
The receptor binding domain (RBD) of SARS-CoV-2 is the primary target of neutralizing
antibodies. We have previously reported the design and characterization of a mammalian …
antibodies. We have previously reported the design and characterization of a mammalian …
Ter‐Seq: A high‐throughput method to stabilize transient ternary complexes and measure associated kinetics
G Chattopadhyay, S Ahmed, NS Srilatha… - Protein …, 2023 - Wiley Online Library
Regulation of biological processes by proteins often involves the formation of transient,
multimeric complexes whose characterization is mechanistically important but challenging …
multimeric complexes whose characterization is mechanistically important but challenging …