Characterization of the Golgi complex cleared of proteins in transit and examination of calcium uptake activities
RS Taylor, SM Jones, RH Dahl… - Molecular biology of …, 1997 - Am Soc Cell Biol
To characterize endogenous molecules and activities of the Golgi complex, proteins in
transit were> 99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment …
transit were> 99% cleared from rat hepatocytes by using cycloheximide (CHX) treatment …
Proinsulin processing in the regulated and the constitutive secretory pathway
PA Halban - Diabetologia, 1994 - Springer
Proinsulin is converted to insulin in betacell granules. Conversion involves endoproteolytic
cleavage at the two pairs of basic residues linking the insulin A-and B-chains to C-peptide …
cleavage at the two pairs of basic residues linking the insulin A-and B-chains to C-peptide …
ATP2C1 gene mutations in Hailey–Hailey disease and possible roles of SPCA1 isoforms in membrane trafficking
M Micaroni, G Giacchetti, R Plebani, GG Xiao… - Cell death & …, 2016 - nature.com
ATP2C1 gene codes for the secretory pathway Ca 2+/Mn 2+-ATPase pump type 1 (SPCA1)
localizing at the golgi apparatus. Mutations on the human ATP2C1 gene, causing decreased …
localizing at the golgi apparatus. Mutations on the human ATP2C1 gene, causing decreased …
Glucose-and GTP-dependent stimulation of the carboxyl methylation of CDC42 in rodent and human pancreatic islets and pure beta cells. Evidence for an essential …
Several GTP-binding proteins (G-proteins) undergo post-translational modifications
(isoprenylation and carboxyl methylation) in pancreatic beta cells. Herein, two of these were …
(isoprenylation and carboxyl methylation) in pancreatic beta cells. Herein, two of these were …
Acid stabilization of insulin
C Bryant, DB Spencer, A Miller, DL Bakaysa… - Biochemistry, 1993 - ACS Publications
Revised Manuscript Received May 18, 1993 abstract: The effect of pH on the conformational
stability of insulin was studied. Surprisingly, the Gibbs free energy of unfolding increased …
stability of insulin was studied. Surprisingly, the Gibbs free energy of unfolding increased …
Protons released during pancreatic acinar cell secretion acidify the lumen and contribute to pancreatitis in mice
N Behrendorff, M Floetenmeyer, C Schwiening… - Gastroenterology, 2010 - Elsevier
BACKGROUND & AIMS: Secretory granules are acidic; cell secretion will therefore lead to
extracellular acidification. We propose that during secretion, protons co-released with …
extracellular acidification. We propose that during secretion, protons co-released with …
Role of furin in granular acidification in the endocrine pancreas: identification of the V-ATPase subunit Ac45 as a candidate substrate
E Louagie, NA Taylor, D Flamez… - Proceedings of the …, 2008 - National Acad Sciences
Furin is a proprotein convertase which activates a variety of regulatory proteins in the
constitutive exocytic and endocytic pathway. The effect of genetic ablation of fur was studied …
constitutive exocytic and endocytic pathway. The effect of genetic ablation of fur was studied …
Similar Peptides from Two β Cell Autoantigens, Proinsulin and Glutamic Acid Decarboxylase, Stimulate T Cells of Individuals at Risk for Insulin-Dependent Diabetes
G Rudy, N Stone, LC Harrison, PG Colman, P McNair… - Molecular …, 1995 - Springer
Background Insulin (1) and glutamic acid decarboxylase (GAD)(2) are both autoantigens in
insulin-dependent diabetes mellitus (IDDM), but no molecular mechanism has been …
insulin-dependent diabetes mellitus (IDDM), but no molecular mechanism has been …
Persistence of coxsackievirus B4 in pancreatic β cells disturbs insulin maturation, pattern of cellular proteins, and DNA methylation
Coxsackievirus-B4 (CV-B4) can persist in pancreatic cell lines and impair the phenoytpe
and/or gene expressions in these cells; however, the models used to study this …
and/or gene expressions in these cells; however, the models used to study this …
Increased secretory demand rather than a defect in the proinsulin conversion mechanism causes hyperproinsulinemia in a glucose-infusion rat model of non-insulin …
C Alarcon, JL Leahy, GT Schuppin… - The Journal of clinical …, 1995 - Am Soc Clin Investig
Hyperproinsulinemia in non-insulin-dependent diabetes mellitus (NIDDM) is due to an
increased release of proinsulin from pancreatic beta cells. This could reside in increased …
increased release of proinsulin from pancreatic beta cells. This could reside in increased …