Fluorogenic labeling strategies have emerged as powerful tools for in vivo and in vitro
imaging applications for diagnostic and theranostic purposes. Free organic chromophores …

Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of
protein dynamics. Recently, fluorogen activating proteins (FAPs)/fluorogen probes for …

The full understanding of cellular functions requires information about protein numbers for
various biomolecular assemblies and their dynamics, which can be partly accessed by …

Simple Summary We comparatively discuss all possible methodologies for the complex
DNA damage in situ detection. Fluorescent microscopy (FM) is the universal approach …

Fluorescent protein-based pH sensors are useful tools for measuring protein trafficking
through pH changes associated with endo-and exocytosis. However, commonly used pH …

In situ investigations in living cell membranes are important to elucidate the dynamic
behaviors of membrane proteins in complex biomembrane environments. Protein-specific …

Herein, we present the synthesis and application of a fluorogenic, large Stokes-shift (> 100
nm), bioorthogonally conjugatable, membrane-permeable tetrazine probe, which can be …

Genetic tags are transformative tools for investigating the function, localization, and
interactions of cellular proteins. Most studies today are reliant on selective labeling of more …

The majority of self-assembled fluorescent dyes suffer from aggregation-caused quenching
(ACQ), which detrimentally affects their diagnostic and therapeutic effectiveness. While …

Highlights•New polymethine cyanine dyes with low melting point designed and
synthesized.•These dyes could be used as fluorescent probes in various biochemical …