CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

Base editors have substantial promise in basic research and as therapeutic agents for the
correction of pathogenic mutations. The development of adenine transversion editors has …

Base editors have been reported to induce off-target mutations in cultured cells, mouse
embryos and rice, but their long-term effects in vivo remain unknown. Here, we develop a …

DNA-editing enzymes perform chemical reactions on DNA nucleobases. These reactions
can change the genetic identity of the modified base or modulate gene expression. Interest …

Mitochondria and chloroplasts are organelles that include their own genomes, which
encode key genes for ATP production and carbon dioxide fixation, respectively. Mutations in …

A fundamental challenge in biology is understanding the molecular details of protein
function. How mutations alter protein activity, regulation, and response to drugs is of critical …

Current DNA base editors contain nuclease and DNA deaminase that enables deamination
of cytosine (C) or adenine (A), but no method for guanine (G) or thymine (T) editing is …

Base editors, including dual base editors, are innovative techniques for efficient base
conversions in genomic DNA. However, the low efficiency of A-to-G base conversion at …

The expansion of the CRISPR-Cas toolbox is highly needed to accelerate the development
of therapies for genetic diseases. Here, through the interrogation of a massively expanded …