Programmable base editors and prime editors offer the potential for highly efficient and
precise genome manipulation. However, in order to safely apply these editors in clinical …

Monogenic disorders refer to a group of human diseases caused by mutations in single
genes. While disease-modifying therapies have offered some relief to symptoms and …

Adenosine-to-inosine tRNA-editing enzyme has been identified for more than two decades,
but the study on its DNA editing activity is rather scarce. We show that amphioxus …

Cytosine base editors (CBEs) are effective tools for introducing C-to-T base conversions, but
their clinical applications are limited by off-target and bystander effects. Through structure …

Base editor (BE) is a gene-editing tool developed by combining the CRISPR/Cas system
with an individual deaminase, enabling precise single-base substitution in DNA or RNA …

Biocatalysis has emerged as a promising alternative to conventional chemical processes for
the production of a wide range of chemicals, providing a sustainable solution to the problem …

Various genome editing tools have been developed for programmable genome
manipulation at specified genomic loci. However, it is crucial to comprehensively interrogate …

The preferred method for disease modeling using induced pluripotent stem cells (iPSCs) is
to generate isogenic cell lines by correcting or introducing pathogenic mutations. Base …

DNA base editors enable direct editing of adenine (A), cytosine (C), or guanine (G), but there
is no base editor for direct thymine (T) editing currently. Here we develop two deaminase …

Base editors enable precise nucleotide changes at targeted genomic loci without requiring
double‐stranded DNA breaks or repair templates. TALE‐adenine base editors (TALE‐ABEs) …