Magnetic separation techniques in sample preparation for biological analysis: a review

J He, M Huang, D Wang, Z Zhang, G Li - Journal of pharmaceutical and …, 2014 - Elsevier
Sample preparation is a fundamental and essential step in almost all the analytical
procedures, especially for the analysis of complex samples like biological and …

Proteomic techniques for characterisation of mesenchymal stem cell secretome

HK Skalnikova - Biochimie, 2013 - Elsevier
Mesenchymal stem cells (MSCs) are multipotent cells with a substantial potential in human
regenerative medicine due to their ability to migrate to sites of injury, capability to suppress …

Purifying biopharmaceuticals: knowledge-based chromatographic process development

AT Hanke, M Ottens - Trends in biotechnology, 2014 - cell.com
The purification of biopharmaceuticals is commonly considered the bottleneck of the
manufacturing process. Increasing product diversity, along with growing regulatory and …

Mass spectrometry–based protein identification in proteomics—a review

Z Noor, SB Ahn, MS Baker… - Briefings in …, 2021 - academic.oup.com
Statistically, accurate protein identification is a fundamental cornerstone of proteomics and
underpins the understanding and application of this technology across all elements of …

Top-down proteomics of large proteins up to 223 kDa enabled by serial size exclusion chromatography strategy

W Cai, T Tucholski, B Chen, AJ Alpert… - Analytical …, 2017 - ACS Publications
Mass spectrometry (MS)-based top-down proteomics is a powerful method for the
comprehensive analysis of proteoforms that arise from genetic variations and post …

Downstream processing of therapeutic peptides by means of preparative liquid chromatography

C De Luca, G Lievore, D Bozza, A Buratti, A Cavazzini… - Molecules, 2021 - mdpi.com
The market of biomolecules with therapeutic scopes, including peptides, is continuously
expanding. The interest towards this class of pharmaceuticals is stimulated by the broad …

Review of three-dimensional liquid chromatography platforms for bottom-up proteomics

VA Duong, JM Park, H Lee - International Journal of Molecular Sciences, 2020 - mdpi.com
Proteomics is a large-scale study of proteins, aiming at the description and characterization
of all expressed proteins in biological systems. The expressed proteins are typically highly …

Deeper protein identification using field asymmetric ion mobility spectrometry in top-down proteomics

VR Gerbasi, RD Melani, SE Abbatiello… - Analytical …, 2021 - ACS Publications
Field asymmetric ion mobility spectrometry (FAIMS), when used in proteomics studies,
provides superior selectivity and enables more proteins to be identified by providing …

Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry

PB Pandeswari, V Sabareesh - RSC advances, 2019 - pubs.rsc.org
Owing to rapid growth in the elucidation of genome sequences of various organisms,
deducing proteome sequences has become imperative, in order to have an improved …

The dynamic state of protein turnover: It's about time

IV Hinkson, JE Elias - Trends in cell biology, 2011 - cell.com
The continual destruction and renewal of proteins that maintain cellular homeostasis has
been rigorously studied since the late 1930s. Experimental techniques for measuring protein …