The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is composed of a repetition
of YSPTSPS heptads and functions as a loading platform for protein complexes that regulate …

Transcription challenges the integrity of replicating chromosomes by generating topological
stress and conflicts with forks,. The DNA topoisomerases Top1 and Top2 and the HMGB …

RNA polymerase III (RNAPIII) synthesizes essential and abundant noncoding RNAs such as
transfer RNAs. Controlling RNAPIII span of activity by accurate and efficient termination is a …

Background The dynamic process of transcription termination produces transient RNA
intermediates that are difficult to distinguish from each other via short-read sequencing …

Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved
hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) …

Single-cell quantification of transcription kinetics and variability promotes a mechanistic
understanding of gene regulation. Here, using single-molecule RNA fluorescence in situ …

The basis of quantitative regulation of gene expression is still poorly understood. In
Arabidopsis thaliana, quantitative variation in expression of FLOWERING LOCUS C (FLC) …

In Saccharomyces cerevisiae, short noncoding RNA (ncRNA) generated by RNA
polymerase II (Pol II) are terminated by the NRD complex consisting of Nrd1, Nab3, and …

Transcription termination delimits transcription units but also plays important roles in limiting
pervasive transcription. We have previously shown that transcription termination occurs …

During transcription initiation, the TFIIH-kinase Kin28/Cdk7 marks RNA polymerase II (Pol II)
by phosphorylating the C-terminal domain (CTD) of its largest subunit. Here we describe a …