BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …

After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse
technique offering exceptional contrast in conjunction with high specificity of labelling with …

Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

Protein adsorption at solid surfaces plays a key role in many natural processes and has
therefore promoted a widespread interest in many research areas. Despite considerable …

Measurement science has been converging to smaller and smaller samples, such that it is
now possible to detect single molecules. This Review focuses on the next generation of …

Optical detection and spectroscopy of single molecules and single nanoparticles have been
achieved at room temperature with the use of surface-enhanced Raman scattering …

We describe a simple illumination method of fluorescence microscopy for molecular
imaging. Illumination by a highly inclined and thin beam increases image intensity and …