Advances in droplet digital polymerase chain reaction on microfluidic chips

D Xu, W Zhang, H Li, N Li, JM Lin - Lab on a Chip, 2023 - pubs.rsc.org
The PCR technique has been known to the general public since the pandemic outbreak of
COVID-19. This technique has progressed through three stages: from simple PCR to real …

Tracing COVID-19 trails in wastewater: A systematic review of SARS-CoV-2 surveillance with viral variants

A Tiwari, S Adhikari, S Zhang, TB Solomon, A Lipponen… - Water, 2023 - mdpi.com
The emergence of new variants of SARS-CoV-2 associated with varying infectivity,
pathogenicity, diagnosis, and effectiveness against treatments challenged the overall …

Unlocking capacities of genomics for the COVID-19 response and future pandemics

S Knyazev, K Chhugani, V Sarwal, R Ayyala, H Singh… - Nature …, 2022 - nature.com
During the COVID-19 pandemic, genomics and bioinformatics have emerged as essential
public health tools. The genomic data acquired using these methods have supported the …

Making waves: Wastewater surveillance of SARS-CoV-2 in an endemic future

F Wu, WL Lee, H Chen, X Gu, F Chandra, F Armas… - Water Research, 2022 - Elsevier
Wastewater-based surveillance (WBS) has been widely used as a public health tool to
monitor the emergence and spread of SARS-CoV-2 infections in populations during the …

Microbiome analysis for wastewater surveillance during COVID-19

KD Brumfield, M Leddy, M Usmani, JA Cotruvo… - MBio, 2022 - Am Soc Microbiol
Wastewater surveillance (WS), when coupled with advanced molecular techniques, offers
near real-time monitoring of community-wide transmission of SARS-CoV-2 and allows …

[HTML][HTML] Normalisation of SARS-CoV-2 concentrations in wastewater: The use of flow, electrical conductivity and crAssphage

J Langeveld, R Schilperoort, L Heijnen… - Science of the Total …, 2023 - Elsevier
Over the course of the Corona Virus Disease-19 (COVID-19) pandemic in 2020–2022,
monitoring of the severe acute respiratory syndrome coronavirus 2 ribonucleic acid (SARS …

Wastewater pandemic preparedness: Toward an end-to-end pathogen monitoring program

JR Clark, A Terwilliger, V Avadhanula… - Frontiers in Public …, 2023 - frontiersin.org
Molecular analysis of public wastewater has great potential as a harbinger for community
health and health threats. Long-used to monitor the presence of enteric viruses, in particular …

Evaluation of the impact of concentration and extraction methods on the targeted sequencing of human viruses from wastewater

M Jiang, ALW Wang, NA Be, N Mulakken… - Environmental …, 2024 - ACS Publications
Sequencing human viruses in wastewater is challenging due to their low abundance
compared to the total microbial background. This study compared the impact of four virus …

Tomato brown rugose fruit virus in aqueous environments–survival and significance of water-mediated transmission

N Mehle, K Bačnik, I Bajde, J Brodarič, A Fox… - Frontiers in plant …, 2023 - frontiersin.org
Tomato brown rugose fruit virus (ToBRFV) has recently emerged as a major disease of
tomatoes and peppers. ToBRFV is a seed-and contact-transmitted virus. In Slovenia …

Wastewater surveillance for SARS-CoV-2 in a small coastal community: Effects of tourism on viral presence and variant identification among low prevalence …

AL Rainey, JC Loeb, SE Robinson, JA Lednicky… - Environmental …, 2022 - Elsevier
Wastewater-based epidemiology has been used to measure SARS-CoV-2 prevalence in
cities worldwide as an indicator of community health, however, few longitudinal studies have …