Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression

D Rizos, A Gutiérrez-Adán… - Biology of …, 2003 - academic.oup.com
We have previously shown that, while the intrinsic quality of the oocyte is the main factor
affecting blastocyst yield during bovine embryo development in vitro, the main factor …

Effect of in vitro culture of human embryos on birthweight of newborns

JC Dumoulin, JA Land, AP Van Montfoort… - Human …, 2010 - academic.oup.com
BACKGROUND In animal models, in vitro culture of preimplantation embryos has been
shown to be a risk factor for abnormal fetal outcome, including high and low birthweight. In …

IVF culture media: past, present and future

E Chronopoulou, JC Harper - Human reproduction update, 2015 - academic.oup.com
BACKGROUND The advances in the world of IVF during the last decades have been rapid
and impressive and culture media play a major role in this success. Until the 1980s fertility …

[PDF][PDF] Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida

TG McEvoy, GD Coull, PJ Broadbent… - Journal of reproduction …, 2000 - Citeseer
Oocytes of all mammals contain an endogenous lipid reserve. This feature reflects their
shared ancestral origin, the yolk-rich amniote egg. However, the lipid is species-specific in …

Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum‐free or serum …

H Abe, S Yamashita, T Satoh… - Molecular reproduction …, 2002 - Wiley Online Library
In this study, the quantitative fluctuation of cytoplasmic lipid droplets (LD) and cryotolerance
were investigated in bovine embryos derived from in vitro‐matured (IVM) and in vitro …

Oocyte maturation: emerging concepts and technologies to improve developmental potential in vitro

RB Gilchrist, JG Thompson - Theriogenology, 2007 - Elsevier
Oocyte in vitro maturation (IVM) is an important reproductive technology that generates
mature oocytes that are capable of supporting preimplantation embryo development and full …

Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes

S Khosla, W Dean, D Brown, W Reik… - Biology of …, 2001 - academic.oup.com
Culture of preimplantation mammalian embryos and cells can influence their subsequent
growth and differentiation. Previously, we reported that culture of mouse embryonic stem …

Simulated physiological oocyte maturation (SPOM): a novel in vitro maturation system that substantially improves embryo yield and pregnancy outcomes

FK Albuz, M Sasseville, M Lane… - Human …, 2010 - academic.oup.com
BACKGROUND Oocyte in vitro maturation (IVM) reduces the need for gonadotrophin-
induced ovarian hyperstimulation and its associated health risks but the unacceptably low …

Current status of embryo technologies in sheep and goat

Y Cognie, G Baril, N Poulin, P Mermillod - Theriogenology, 2003 - Elsevier
This review presents an overview of the technical bases of in vivo and in vitro embryo
production in sheep and goat. The current limitations of in vivo production, such as variability …

Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior

R Fernández-Gonzalez, P Moreira… - Proceedings of the …, 2004 - National Acad Sciences
The long-term developmental and behavioral consequences of mammalian embryo culture
are unknown. By altering the culture medium with the addition of FCS, we wanted to …