Protein aggregation is the main hallmark of neurodegenerative diseases. Many proteins
found in pathological inclusions are known to undergo liquid-liquid phase separation, a …

Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …

The development of the TMTpro-16plex series expanded the breadth of commercial isobaric
tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described …

Protein ubiquitination is a dynamic multifaceted post-translational modification involved in
nearly all aspects of eukaryotic biology. Once attached to a substrate, the 76-amino acid …

Regulatory protein phosphorylation controls normal and pathophysiological signaling in
eukaryotic cells. Despite great advances in mass-spectrometry-based proteomics, the …

Mitochondria are dynamic, multifunctional cellular organelles that play a fundamental role in
maintaining cellular homeostasis. Keeping the quality of mitochondria in check is of …

Ubiquitin-specific protease 14 (USP14), a deubiquitinating enzyme (DUB), is associated
with proteasomes and exerts a dual function in regulating protein degradation. USP14 …

Cystathionine-β-synthase (CBS), the first (and rate-limiting) enzyme in the transsulfuration
pathway, is an important mammalian enzyme in health and disease. Its biochemical …

Genome-wide association studies (GWAS) have identified the PICALM (Phosphatidylinositol
binding clathrin-assembly protein) gene as the most significant genetic susceptibility locus …

Cells have a remarkable ability to synthesize large amounts of protein in a very short period
of time. Under these conditions, many hydrophobic surfaces on proteins may be transiently …