Establishment of production platforms through prokaryotic engineering in microbial
organisms would be one of the most efficient means for chemicals, protein, and biofuels …

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)
system has been rapidly developed as versatile genomic engineering tools with high …

CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …

Finding efficient therapeutic strategies to fight antibiotic-resistant bacteria is a complicated
affair specifically in the therapy of chronic bacterial infections related to hospital-acquired …

Bacterial cells are widely used to produce value-added products due to their versatility, ease
of manipulation, and the abundance of genome engineering tools. However, the efficiency of …

Class 2 CRISPR–Cas proteins have been widely developed as genome editing and
transcriptional regulating tools. Class 1 type I CRISPR–Cas constitutes~ 60% of all the …

Genome minimization is an effective way for industrial chassis development. In this study,
Zymomonas mobilis ZMNP, a plasmid-free mutant strain of Z. mobilis ZM4 with four native …

Inhibitors in lignocellulosic hydrolysates are toxic to Zymomonas mobilis and reduce its
bioethanol production. This study revealed cysteine supplementation enhanced furfural …

A highly effective metabolic pathway is the key for an efficient cell factory. However, the
engineered homologous or heterologous multi-gene pathway may be unbalanced …

Bacillus licheniformis DW2 is an important industrial strain for bacitracin production, and it is
also used for biochemical production, however, the lack of effective toolkit for precise …