Measurements of the thermodynamic properties of biomolecular folding (Δ G°, Δ H°, Δ S°,
etc.) provide a wealth of information on the folding process and have long played a central …

Current methods for the determination of molecular interactions are widely used in the
analytical sciences. To identify new methods, we investigated as a model system the …

Single-molecule fluorescence imaging is a powerful method to measure reversible reaction
kinetics, allowing one to monitor the bound state of individual probe molecules with …

In this paper review, some of the important characterizations and mechanisms in the
chemistry of cyanine dyes were explained. This include topics like absorption spectral …

To understand the complex fluorescence properties of astraphloxin (CY3)-labelled
oligonucleotides, it is necessary to take into account the redox properties of the …

Background MicroRNAs are small noncoding RNAs about 22 nt long that play key roles in
almost all biological processes and diseases. The fluorescent labeling and lipofection are …

Recently, nucleic acid amplification and detection techniques have progressed based on
advances in in microfluidics, microelectronics, and optical systems. Nucleic acids …

For the first time we used a CuAAC click reaction for the synthesis of cyanine labeled qPCR
probes. We performed the comparison of dyes and quenchers (Cy5 vs. disulfo–Cy5 and …

We report the synthesis and site-specific incorporation in oligodeoxynucleotides (ODNs) of
an emissive deoxyuridine analog electronically conjugated on its C5-position with a 3 …

A synthesis of the carbopyronine dye Carboxy ATTO 647N from simple materials is reported.
This route proceeds in 11 forward steps from 3-bromoaniline with the key xanthone …