[HTML][HTML] Optimisation and standardisation of a multiplex immunoassay of diverse Plasmodium falciparum antigens to assess changes in malaria transmission using …

L Wu, T Hall, I Ssewanyana, T Oulton… - Wellcome open …, 2019 - ncbi.nlm.nih.gov
Background: Antibody responses have been used to characterise transmission and
exposure history in malaria-endemic settings for over a decade. Such studies have typically …

Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

X Martiáñez-Vendrell, A Jiménez, A Vásquez… - Malaria journal, 2020 - Springer
Background Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the
qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and …

Plasmodium falciparum and helminth coinfections increase IgE and parasite-specific IgG responses

R Santano, R Rubio, B Grau-Pujol, V Escola… - Microbiology …, 2021 - Am Soc Microbiol
Coinfection with Plasmodium falciparum and helminths may impact the immune response to
these parasites because they induce different immune profiles. We studied the effects of …

Framework for Characterizing Longitudinal Antibody Response in Children After Plasmodium falciparum Infection

E Rogier, D Nace, PR Dimbu, B Wakeman… - Frontiers in …, 2021 - frontiersin.org
Human Plasmodium infection produces a robust adaptive immune response. Time courses
for 104 children followed for 42 days after initiation of Plasmodium falciparum chemotherapy …

Analysis of factors affecting the variability of a quantitative suspension bead array assay measuring IgG to multiple Plasmodium antigens

I Ubillos, R Aguilar, H Sanz, A Jiménez, M Vidal… - PLoS …, 2018 - journals.plos.org
Reducing variability of quantitative suspension array assays is key for multi-center and large
sero-epidemiological studies. To maximize precision and robustness of an in-house IgG …

Serological profiling for malaria surveillance using a standard ELISA protocol

LM Murungi, RK Kimathi, J Tuju, G Kamuyu… - Malaria Control and …, 2019 - Springer
The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost
method for measuring soluble ligands such as antibodies and proteins in biological …

Multiplex Assays for Analysis of Antibody Responses to South Asian Plasmodium falciparum and Plasmodium vivax Malaria Infections

EO Momoh, SK Ghag, J White, DG Mudeppa… - Vaccines, 2023 - mdpi.com
Malaria remains a major global health challenge, causing over 0.6 million yearly deaths. To
understand naturally acquired immunity in adult human populations in malaria-prevalent …

[HTML][HTML] VAR2CSA serology to detect Plasmodium falciparum transmission patterns in pregnancy

AM Fonseca, R Gonzalez, A Bardaji… - Emerging Infectious …, 2019 - ncbi.nlm.nih.gov
Pregnant women constitute a promising sentinel group for continuous monitoring of malaria
transmission. To identify antibody signatures of recent Plasmodium falciparum exposure …

VAR2CSA antibodies in non-pregnant populations

S Gnidehou, SK Yanow - Trends in parasitology, 2021 - cell.com
The Plasmodium falciparum protein VAR2CSA is a critical mediator of placental malaria,
and VAR2CSA antibodies (IgGs) are important to protect pregnant women. Although …

Dried blood spots: a robust tool for malaria surveillance in countries targeting elimination

M Nain, A Sinha, A Sharma - Journal of Vector Borne Diseases, 2023 - journals.lww.com
With the advancements in analytical and molecular techniques, Dried Blood Spots (DBS)
are re-emerging as attractive and cost-effective alternatives for global health surveillance …